Development of Streptococcus thermophilus lacZ as a reporter gene for Candida albicans.
Development of Streptococcus thermophilus lacZ as a reporter gene for Candida albicans.
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DOI：
10.1099/00221287-147-5-1189
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发表时间：
2001-05
期刊：
影响因子：
1.5
通讯作者：
M. Uhl;A. D. Johnson;M. Uhl;A. D. Johnson;Larry Mckay;Doug Kellogg
中科院分区：
文献类型：
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作者：
M. Uhl;A. D. Johnson;M. Uhl;A. D. Johnson;Larry Mckay;Doug Kellogg
报告基因的发展促进了许多生物基因调控的研究。作者报道了利用嗜热链球菌（一种编码β -半乳糖苷酶的基因）中的lacZ作为真菌病原体白色念珠菌的报告基因。作为测试用例，链球菌。将嗜热菌lacZ置于三种不同的白色念珠菌启动子的控制下：MAL2（麦芽糖酶），由麦芽糖诱导；HWP1（菌丝细胞壁蛋白），由促进丝状细胞生长的条件诱导；ACT1（肌动蛋白）。这些构建体都被整合到白色念珠菌基因组中，β -半乳糖苷酶活性很容易从这些菌株中检测到，但只有在适当的生长条件下。β -半乳糖苷酶活性可通过几种方法检测：使用渗透细胞进行定量液体测定，将菌落复制到纸滤镜上进行比色测定，以及在含有指示剂X-Gal的培养基上生长的菌落进行原位显色。这些结果表明，STREP：嗜热菌lacZ作为这种医学上重要的酵母基因调控的监视器是有用的。
The study of gene regulation in many organisms has been facilitated by the development of reporter genes. The authors report the use of lacZ from Streptococcus thermophilus, a gene encoding a beta-galactosidase, as a reporter for the fungal pathogen Candida albicans. As test cases, Strep. thermophilus lacZ was placed under control of three different C. albicans promoters: MAL2 (maltase), inducible by maltose; HWP1 (hyphal cell wall protein), induced by conditions that promote filamentous growth; and ACT1 (actin). These constructs were each integrated into the C. albicans genome and beta-galactosidase activity was readily detected from these strains, but only under the appropriate growth conditions. Beta-galactosidase activity could be detected by several methods: quantitative liquid assays using permeabilized cells, colorimetric assays of colonies replicated to paper filters, and in situ coloration of colonies growing on medium containing the indicator X-Gal. These results show the usefulness of STREP: thermophilus lacZ as a monitor of gene regulation in this medically important yeast.