Genetic Organization in CAENORHABDITIS ELEGANS: Fine-Structure Analysis of the unc-22 Gene.
Genetic Organization in CAENORHABDITIS ELEGANS: Fine-Structure Analysis of the unc-22 Gene.
复制标题
秀丽隐杆线虫的遗传组织：unc-22 基因的精细结构分析。
作者：
D. Moerman;D. Baillie
秀丽隐杆线虫unc-22基因的精细结构分析揭示了许多可通过重组分离的位点。分离出8个新的甲基磺酸乙酯诱导的unc-22基因隐性突变。利用这些新的等位基因，以及e66，许多可分离的位点已经被确定并相对定位。所获得的图谱距离与黑腹果蝇基因内重组相关的图谱距离相当，表明遗传精细结构分析在秀丽隐杆线虫中是可行的。提出了可能的基因转换的证据。初步估计unc-22基因大小为2.4 x 10（-2）个图谱单位。
Fine-structure analysis of the unc-22 gene of Caenorhabditis elegans has revealed a number of sites that are separable by recombination. Eight new ethyl methanesulfonate-induced recessive mutations of the unc-22 gene have been isolated. Using these new alleles, as well as e66, a number of separable sites have been identified and positioned relative to one another. The map distances obtained are found to be comparable to those associated with intragenic recombination in Drosophila melanogaster, indicating that genetic fine-structure analysis is feasible in Caenorhabditis elegans. Evidence of possible gene conversion is presented. A preliminary estimate of the unc-22 gene size is 2.4 x 10(-2) map units.