Quantitative assessment of developing afferent patterns in the cat inferior colliculus revealed with calbindin immunohistochemistry and tract tracing methods
Quantitative assessment of developing afferent patterns in the cat inferior colliculus revealed with calbindin immunohistochemistry and tract tracing methods
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DOI：
10.1016/j.neuroscience.2005.03.020
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
McHaffie, JG
中科院分区：
文献类型：
--
作者：
Henkel, CK;Gabriele, ML;McHaffie, JG
下丘中央核（CNIC）由一系列有序的纤维树突层组成。这些层包括用于多达 13 个不同的上行听觉通路的集成电路，每个通路都按音调排序。钙结合蛋白，例如 calbindinD28k (CB)，可能是有用的神经化学标记物，用于这些层中传入输入的特定子集及其受发育调节的空间组织。在本研究中，使用 CB 免疫组织化学检查出生后 1-42 天的小猫和成年猫 CNIC，并使用顺行示踪剂标记相似年龄时从外侧上橄榄核 (LSO) 到 CNIC 的传入投射。描述了 CB 免疫阳性的独特轴突丛。这种 CB 传入区室在出生时就存在，并在所检查的整个年龄段中持续存在。在出生时，小猫 CNIC 的 CB 免疫染色神经丛就组织成离散的带，厚度约为 75 μm，长 500 μm。在成年 CNIC 中，CB 免疫染色纤维的周期性带状图案与小猫相似，尽管带更厚（145 μm）和更长（700 μm）。成年猫条带厚度的增长似乎与 IC 的增长成正比，而密集的 CB 免疫染色条带的长度在某种程度上更集中在纤维树突层的中心区域。 CB 免疫染色丛的带状图案与用羰花青染料、1,1'-二十八烷基-3,3,3',3'-四甲基吲哚碳花青高氯酸盐标记的新生小猫和用辣根过氧化物酶缀合的麦芽凝集素标记的成年猫中 LSO 传入投射的位置和尺寸密切相关。结果揭示了 CNIC 层中一种类型的突触区室（条带）的神经化学标记，这种突触区室在小猫出现听力之前就组织起来，但可能会经历一些出生后的修剪。 (c) 2005 年由 Elsevier Ltd 代表 IBRO 出版。
The central nucleus of the inferior colliculus (CNIC) is comprised of an orderly series of fibrodendritic layers. These layers include integrative circuitry for as many as 13 different ascending auditory pathways, each tonotopically ordered. Calcium-binding proteins, such as calbindinD28k (CB), may be useful neurochemical markers for specific subsets of afferent input in these layers and their spatial organization that are developmentally regulated. In this study, CB-immunohistochemistry was used to examine 1-42 postnatal-day-old kitten and adult cat CNIC and anterograde tracers were used to label afferent projections from the lateral superior olivary nucleus (LSO) to the CNIC at similar ages. A distinct axonal plexus that is CB-immunopositive is described. This CB-afferent compartment is present at birth and persists throughout the ages examined. Already at birth, the CB-immunostained plexus in kitten CNIC is organized into discrete bands that are approximately 75 mu m thick and 500 mu m long. In adult CNIC, the periodic banded pattern of CB-immunostained fibers is similar to that in kittens albeit bands are thicker (145 mu m) and longer (700 mu m). Growth in band thickness in adult cat appears proportional to growth of the IC, whereas length of the dense CB-immunostained bands is somewhat more focused in the central region of fibrodendritic layers. The banded pattern of the CB-immunostained plexus is well correlated with the location and dimension of afferent projections from the LSO in newborn kitten labeled with carbocyanine dye, 1,1'-dioctodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate and in adult cat labeled with wheat germ agglutinin conjugated with horseradish peroxidase. The results reveal a neurochemical marker for one type of synaptic compartment in CNIC layers, banding, that is organized before hearing onset in kittens, but that may undergo some postnatal pruning. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.