INHIBITION OF CHONDROGENESIS BY RETINOIC ACID IN LIMB MESENCHYMAL CELLS-INVITRO - EFFECTS ON PGE2 AND CYCLIC-AMP CONCENTRATIONS
INHIBITION OF CHONDROGENESIS BY RETINOIC ACID IN LIMB MESENCHYMAL CELLS-INVITRO - EFFECTS ON PGE2 AND CYCLIC-AMP CONCENTRATIONS
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DOI：
10.1016/0922-3371(88)90056-1
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发表时间：
1988-09-01
期刊：
影响因子：
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通讯作者：
SAWYER, LM
中科院分区：
文献类型：
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作者：
BIDDULPH, DM;DOZIER, MM;SAWYER, LM
在高密度微团培养的鸡肢芽间充质细胞中，研究了维甲酸（RA）对前列腺素E_2（PGE_2）和环磷酸腺苷（cAMP）浓度的影响。在培养的最初24小时期间，将细胞暴露于0.05-1.0 μ g/ml浓度的RA以剂量依赖性方式抑制软骨形成，1.0 μ g/ml完全抑制软骨形成。PGE 2和cAMP的浓度增加在prechondrogenic期间在对照细胞中以密切相关的方式，并保持在整个6天期间检查升高。RA（0.05和0.5 μ g/ml）的加入在任何时间点都没有显著改变cAMP浓度，但在6天培养物中以浓度依赖性方式相对于对照细胞显著升高了PGE 2水平。添加二丁酰环腺苷酸增强软骨形成在对照细胞之间的第3和第4天，但未能改变RA对软骨形成的抑制作用。结果表明，虽然PGE 2和cAMP是软骨分化的重要信号，但RA对这一过程的抑制作用是通过其他机制介导的。
Effects of retinoic acid (RA) on prostaglandin E2 (PGE2) and cyclic AMP (cAMP) concentrations were investigated in high density, micromass cultures of mesenchymal cells derived from chick limb buds. Exposure of cells during the initial 24 h of culture to RA concentrations between 0.05-1.0 .mu.g/ml inhibited chondrogenesis in a dose-dependent manner with 1.0 .mu.g/ml totally inhibiting cartilage formation. Concentrations of PGE2 and cAMP increased during the prechondrogenic period in control cells in a closely related way and remained elevated throughout the six-day period examined. Addition of RA (0.05 and 0.5 .mu.g/ml) did not significantly alter cAMP concentrations at any time point, but significantly elevated PGE2 levels relative to control cells in six-day cultures in a concentration-dependent manner. Addition of dibutyryl cAMP enhanced chondrogenesis in control cells between days 3 and 4, but failed to alter the inhibitory effect of RA on chondrogenesis. The results indicate that while, PGE2 and cAMP are important signals in cartilage differentiation, the inhibitory effects of RA on this process are mediated through some other mechanism.