Azaphilones biosynthesis complements the defence mechanism ofTrichoderma guizhouenseagainst oxidative stress
Azaphilones biosynthesis complements the defence mechanism ofTrichoderma guizhouenseagainst oxidative stress
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Azaphilones 生物合成补充了贵州木霉对抗氧化应激的防御机制。
DOI：
10.1111/1462-2920.15246
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发表时间：
2020-10-19
影响因子：
5.1
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
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作者：
Pang, Guan;Sun, Tingting;Shen, Qirong
丝状真菌是已知的大量不同次生代谢物(SMS)的生产者，这些物质有助于确保它们的环境利基。在这里，我们证明了SMS在真菌防御其他真菌方面具有额外的作用：木霉是一种真菌寄生虫，能够拮抗尖孢镰刀菌。Sp.cubenserace 4(FOC4)，形成气生菌丝，用过氧化氢杀死寄主。同时，由两个聚酮合成酶组成的基因簇也得到了强烈的表达。利用功能遗传学，我们对这一簇进行了表征，并将其产物鉴定为氮杂环丙酮(称为三氮杂氮杂环丙酮)。结果表明，三氮卓酮类化合物无抗真菌毒性，但具有较强的清除自由基活性。在不同的氧化应激条件下，三氮卓酮类化合物的抗氧化性具有活体功能。因此，我们得出结论，三氮杂环酮的生物合成是一种互补的抗氧化机制，并保护T。它产生的过氧化氢是用来对抗Foc4等其他真菌的。
Filamentous fungi are known as producers of a large array of diverse secondary metabolites (SMs) that aid in securing their environmental niche. Here, we demonstrated that the SMs have an additional role in fungal defence against other fungi:Trichoderma guizhouense, a mycoparasite, is able to antagonizeFusarium oxysporumf. sp.cubenserace 4 (Foc4) by forming aerial hyphae that kill the host with hydrogen peroxide. At the same time, a gene cluster comprising two polyketide synthases is strongly expressed. Using functional genetics, we characterized this cluster and identified its products as azaphilones (termed as trigazaphilones). The trigazaphilones were found lacking of antifungal toxicity but exhibited high radical scavenging activities. The antioxidant property of trigazaphilones wasin vivofunctional under various tested conditions of oxidative stress. Thus, we conclude that the biosynthesis of trigazaphilones serves as a complementary antioxidant mechanism and defendsT. guizhouenseagainst the hydrogen peroxide that it produces to combat other fungi like Foc4.