Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation.
Peroxidation due to cryoprotectant treatment is a vital factor for cell survival in Arabidopsis cryopreservation.
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DOI：
10.1016/j.plantsci.2013.07.011
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发表时间：
2013-11
期刊：
影响因子：
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通讯作者：
L. Ren;Di Zhang;Xiang-Ning Jiang;Y. Gai;Wei-ming Wang;B. Reed;Xiao-hui Shen
中科院分区：
文献类型：
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作者：
L. Ren;Di Zhang;Xiang-Ning Jiang;Y. Gai;Wei-ming Wang;B. Reed;Xiao-hui Shen
冷冻保存可以成为长期储存植物种质的安全且经济有效的工具。在拟南芥中，随着生长的进展，从低温处理中恢复的能力逐渐丧失。低温处理后，48小时幼苗即可恢复生长，而72小时幼苗则死亡。为什么苗龄和成活率呈负相关是一个有趣的问题。进行比较转录组学以筛选暴露于冷冻保护剂后 48 小时和 72 小时幼苗之间的差异表达基因。在差异表达基因中，氧化应激反应基因在冷冻保护剂处理中发挥重要作用，而过氧化是与细胞存活相关的关键因素。幼苗在 72 小时比 48 小时经历更多的过氧化。综合分析表明，过氧化损伤膜系统导致光磷酸化和氧化磷酸化损伤。此外，在拟南芥幼苗的低温处理中发现了类似细胞凋亡的事件。 48小时和72小时的幼苗在冷冻保护剂处理过程中发生了不同程度的膜脂过氧化，减少氧化应激损伤是成功冷冻保存的重要因素。该研究为低温保存中的遗传调控机制提供了新的见解，并建立了一个很好的模型来测试和评估外源抗氧化剂和常规低温保护剂在植物低温保存中的效果。
Cryopreservation can be a safe and cost-effective tool for the long-term storage of plant germplasm. InArabidopsis, the ability to recover from cryogenic treatment was lost as growth progressed. Growth could be restored in 48-h seedlings, whereas 72-h seedlings died after cryogenic treatment. Why seedling age and survival are negatively correlated is an interesting issue. A comparative transcriptomics was performed to screen differentially expressed genes between 48- and 72-h seedlings after exposure to cryoprotectant. Among differentially expressed genes, oxidative stress response genes played important roles in cryoprotectant treatment, and peroxidation was a key factor related to cell survival. Seedlings underwent more peroxidation at 72-h than at 48-h. A comprehensive analysis indicated that peroxidation injured membrane systems leading to photophosphorylation and oxidative phosphorylation damage. Furthermore, the apoptosis-like events were found in cryogenic treatment ofArabidopsisseedlings. 48- and 72-h seedlings underwent different degrees of membrane lipid peroxidation during cryoprotectant treatment, and reducing the injury of oxidative stress was an important factor to successful cryopreservation. This study provided a novel insight of genetic regulatory mechanisms in cryopreservation, and established an excellent model to test and evaluate the effect of exogenous antioxidants and conventional cryoprotectants in plant cryopreservation.