Studies on pure red cell aplasia. VI. Development of two-stage erythroblast cytotoxicity method and role of complement.
Studies on pure red cell aplasia. VI. Development of two-stage erythroblast cytotoxicity method and role of complement.
复制标题
纯红细胞再生障碍性贫血的研究。
DOI：
10.5555/uri:pii:002221437390022x
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
S. Krantz
中科院分区：
文献类型：
--
作者：
S. Zaentz;S. Krantz
用~（59）Fe与人骨髓细胞孵育21小时，产生~（59）Fe标记的成红细胞。当这些标记的细胞与6个纯红细胞再生障碍性贫血（PRCA）血浆孵育时，3个产生增加的59 Fe从成红细胞释放到上清液培养基中。59 Fe的释放增加先前已被证明代表血红蛋白的释放，并且是成红细胞细胞毒性的指标。为了表征PRCA等离子体的效果，采用了两阶段孵育方法。使用该方法，7个PRCA等离子体中的4个产生了59 Fe的增加释放。11例多输血患者中有10例PRCA以外的疾病患者的血浆未增加59 Fe的释放，高于正常水平。使用两阶段方法，已经表明在第一阶段骨髓细胞与PRCA γ G-球蛋白接触是必要的，在第二阶段细胞与新鲜冷冻的正常血浆接触是必要的。第二阶段正常血浆在56 ℃下灭活。热处理和用苏拉明、酵母聚糖和乙二醇四乙酸（EGTA）（3种补体抑制剂）处理，表明辅因子可能是补体。γ G-球蛋白组分中存在细胞毒性因子及其对补体的明显依赖性表明其可能是一种抗体或免疫复合物。
Abstract Human marrow cells were incubated with 59 Fe for 21 hours to produce 59 Fe-labeled erythroblasts. When these labeled cells were incubated with 6 pure red cell aplasia (PRCA) plasmas, 3 produced an increased release of 59 Fe from the erythroblasts into the supernatant media. The increased release of 59 Fe has previously been shown to represent a release of hemoglobin and is an index of erythroblast cytotoxicity. To characterize the effect of the PRCA plasmas a two-stage incubation method was adopted. Using this method 4 of 7 PRCA plasmas produced an increased release of 59 Fe. The plasmas of 10 of 11 polytransfused patients with diseases other than PRCA did not increase the release of 59 Fe above the normal level. Using the two-stage method it has been shown that marrow cell contact with PRCA γG-globulins is necessary in the first stage, and cell contact with fresh frozen normal plasma is necessary in the second stage. The second-stage normal plasma is inactivated by 56 °C. heat-treatment and by treatment with suramin, zymosan, and ethylene-glycol-tetra-acetic-acid (EGTA), 3 inhibitors of complement, indicating that the cofactor may be complement. The presence of the cytotoxic factor in the γG-globulin fraction and its apparent dependence on complement indicate that it may be an antibody or an immune complex.