Localization of heterogeneous nuclear ribonucleoprotein in the interphase nuclear matrix core filaments and on perichromosomal filaments at mitosis.
Localization of heterogeneous nuclear ribonucleoprotein in the interphase nuclear matrix core filaments and on perichromosomal filaments at mitosis.
复制标题
有丝分裂时异质核核糖核蛋白在间期核基质核心丝和染色体周丝中的定位。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
S. Penman
中科院分区：
文献类型：
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作者：
Dacheng He;Terence MARTINt;S. Penman
虽然异质性核RNA(HnRNA)已经定位于核基质的核心细丝亚结构，但其在细丝网络中的确切位置尚不清楚。FA12单抗可以高分辨率定位hN核糖核蛋白(HnRNPs)，推测也可以定位hnRNA。无树脂电子显微镜切片的金珠免疫标记显示，fA12抗原存在于纤维颗粒材料中，包裹在纤维网络中，而不是纤维本身。有丝分裂时，hnRNP抗原分散成围绕着染色体和纺锤体极的光晕。免疫金染色示fA12染色的纤维颗粒物质与染色体周围和着丝粒周围的纤维相联系，与有丝分裂的纺锤体纤维不同。FA12还标记了胞质分裂后残留的中体。
Although heterogeneous nuclear RNA (hnRNA) has been localized to the core filament substructure of the nuclear matrix, its precise location in the filament network has been unknown. The fA12 monoclonal antibody can localize, at high resolution, hn ribonucleoproteins (hnRNPs) and, presumably, hnRNA. Gold bead immunolabeling of resinless electron microscopy sections showed the fA12 antigens were in the fibrogranular material enmeshed in the filament network and not in the filaments themselves. At mitosis, hnRNP antigens became dispersed into a halo surrounding the chromosomes and spindle poles. Immunogold staining showed fA12 stained fibrogranular material associated with perichromosomal and pericentriolar filaments distinct from the mitotic spindle fibers. fA12 also labeled the midbody remaining after cytokinesis.