MYT1 - A MEMBRANE-ASSOCIATED INHIBITORY KINASE THAT PHOSPHORYLATES CDC2 ON BOTH THREONINE-14 AND TYROSINE-15
MYT1 - A MEMBRANE-ASSOCIATED INHIBITORY KINASE THAT PHOSPHORYLATES CDC2 ON BOTH THREONINE-14 AND TYROSINE-15
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DOI：
10.1126/science.270.5233.86
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发表时间：
1995-10-06
期刊：
影响因子：
56.9
通讯作者：
DUNPHY, WG
中科院分区：
文献类型：
--
作者：
MUELLER, PR;COLEMAN, TR;DUNPHY, WG
Cdc2是一种依赖细胞周期蛋白的激酶，它控制细胞进入有丝分裂。Cdc2的苏氨酸 - 14和酪氨酸 - 15位点的磷酸化会抑制该酶的活性，并防止有丝分裂过早启动。尽管在各种生物中已确定Wee1是使酪氨酸 - 15磷酸化的激酶，但苏氨酸 - 14特异性激酶尚未被分离出来。从非洲爪蟾中克隆出了一种互补DNA，它编码Myt1，Myt1是Wee1家族的一个成员，被发现能有效地使Cdc2的苏氨酸 - 14和酪氨酸 - 15磷酸化。Myt1是一种膜相关蛋白，包含一个假定的跨膜区段。免疫耗竭研究表明，Myt1是非洲爪蟾卵提取物中主要的苏氨酸 - 14特异性激酶。Myt1的活性在细胞周期中受到高度调控，这表明Wee1的这个相关蛋白在有丝分裂控制中起作用。
Cdc2 is the cyclin-depefident kinase that controls entry of cells into mitosis. Phosphorylation of Cdc2 on threonine-14 and tyrosine-15 inhibits the activity of the enzyme and prevents premature initiation of mitosis. Although Wee1 has been identified as the kinase that phosphorylates tyrosine-15 in various organisms, the threonine-14-specific kinase has not been isolated. A complementary DNA was cloned from Xenopus that encodes Myt1, a member of the Wee1 family that was discovered io phosphorylate Cdc2 efficiently on both threonine-14 and tyrosine-15. Myt1 is a membrane-associated protein that contains a putative transmembrane segment. Immunodepletion studies suggested that Myt1 is the predominant threonine-14-specific kinase in Xenopus egg extracts, Myt1 activity is highly regulated during the cell cycle, suggesting that this relative of Wee1 plays a role in mitotic control.