The positive effects of zinc on skeletal strength in growing rats
The positive effects of zinc on skeletal strength in growing rats
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DOI：
10.1016/s8756-3282(01)00616-0
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发表时间：
2001-12-01
期刊：
影响因子：
4.1
通讯作者：
Mosekilde, L
中科院分区：
文献类型：
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作者：
Ovesen, J;Moller-Madsen, B;Mosekilde, L
本研究的目的是评估营养性锌缺乏和补充剂在完整的动物模型，生长大鼠骨骼的影响。本研究计划为剂量-反应研究。将36只4周龄雄性Wistar大鼠分为3组，每组12只。大鼠可以自由摄入添加不同锌量的半合成饲料。第1组为不含锌饲料（含锌2 mg/kg），第2组为正常锌饲料（含锌47 mg/kg），第3组为补锌饲料（含锌60 mg/kg）。实验开始后4周处死所有动物，取出右侧股骨。在以下骨骼部位测量生物力学效应：股骨干、股骨颈和股骨远端干骺端。此外，在骨干中段区域进行静态组织形态测定。生物力学测试显示，在所有研究部位，锌诱导的骨强度显著增加。它还表明，锌以剂量依赖性的方式影响骨强度，除了在远端干骺端，其中正常锌饮食组和高锌饮食组之间没有显着差异。锌还提高了大鼠的生长速度。体重和股骨长度呈剂量依赖性增加。静态组织形态计量学表明，锌发挥其主要作用的骨膜信封，从而增加骨面积，组织面积和轴向惯性矩。我们的结论是，营养补充锌生长大鼠诱导增加股骨颈和股骨干的骨强度。这些结果进一步支持锌对骨代谢具有积极作用的观点，其模拟生长激素（GH）或胰岛素样生长因子1（IGF-1）的作用。(C)2001年，Elsevier Science Inc. All rights reserved.
The aim of the present study was to assess the skeletal effects of alimentary zinc depletion and supplementation in an animal model of intact, growing rats. The study was planned as a dose-response study. Thirty-six male Wistar rats, 4 weeks old, were divided into three groups of 12 rats each. The rats had free access to a semisynthetic diet with different amounts of zinc added. Group I was given a zinc-free diet containing 2 mg zinc/kg, group 2 was given a normal-zinc diet containing 47 mg zinc/kg; and group 3 was given a zinc-supplemented diet containing 60 mg zinc/kg. All animals were killed 4 weeks after initiation of the experiment and the right femora were removed. The biomechanical effects were measured at the following skeletal sites: femoral diaphysis; femoral neck; and distal femoral metaphysis. In addition, static histomorphometry was performed at the middiaphyseal region. Biomechanical testing revealed a significant zinc-induced increase in bone strength at all sites investigated. It also showed that zinc influenced bone strength in a dose-dependent manner except at the distal metaphysis, where there was no significant difference between the group fed normal-zinc diet and the group fed a hyper-zinc diet. Zinc also improved the rates of growth in the rats. The body weights and length of femora increased dose-dependently. Static histomorphometry showed that zinc exerted its main effect on the periosteal envelope, thereby increasing bone area, tissue area, and axial moment of inertia. We conclude that alimentary zinc supplementation in growing rats induces an increase of bone strength in both the femoral neck and the femoral diaphysis. These results further support the view that zinc has a positive effect on bone metabolism which mimicks that of growth hormone (GH) or insulin-like growth factor 1 (IGF-1). (C) 2001 by Elsevier Science Inc. All rights reserved.