Preventive effects of oleuropein against cardiac remodeling after myocardial infarction in Wistar rat through inhibiting angiotensin-converting enzyme activity
Preventive effects of oleuropein against cardiac remodeling after myocardial infarction in Wistar rat through inhibiting angiotensin-converting enzyme activity
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DOI：
10.3109/15376516.2015.1053648
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发表时间：
2015-01-01
影响因子：
3.2
通讯作者：
Allouche, Noureddine
中科院分区：
文献类型：
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作者：
Mnafgui, Kais;Khlif, Ines;Allouche, Noureddine
目的：心肌梗死仍然是全球心血管疾病死亡的主要原因。本研究旨在评估橄榄苦苷对减轻异丙肾上腺素诱发的大鼠心肌梗死心脏重塑的保护作用。 方法和结果：将雄性Wistar大鼠随机分为四组，对照组、异丙肾上腺素（Isop）组和预先口服两种不同剂量（20和40 mg/kg）橄榄苦苷的动物7天，并用异丙肾上腺素中毒。 (Isop+Oleu20)和(Isop+Oleu40)组。连续两天向未经治疗的大鼠皮下注射异丙肾上腺素（100毫克/千克体重），结果显示ST段抬高、心脏重量指数以及心电图模式和血流动力学功能显着增加。另外，与正常大鼠相比，Isop组血清中心肌肌钙蛋白-T、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）和丙氨酸转氨酶（ALT）的血清水平显着升高（分别为345%、82%、73%和106%）。异丙肾上腺素诱导的心肌损伤表现为血清脂质谱的改变，胰脂肪酶活性增加 94%，血管紧张素转换酶 (ACE) 活性增加 78%，这反映了心脏重塑过程的发生。梗死组的组织病理学结果显示心肌坏死和细胞炎症浸润。而橄榄苦苷治疗可通过降低心脏损伤标志物特别是肌钙蛋白-T、恢复血流动力学参数以及通过抑制ACE活性来减弱心脏重塑过程，从而对心肌产生良好的保护作用。结论：橄榄苦苷对急性心肌梗死大鼠的心脏重塑过程具有良好的预防作用。
Objective: Myocardial infarction remains the major cause of global death due to cardiovascular diseases. This study aimed to assess the protective role of oleuropein in attenuating the cardiac remodeling in isoproterenol-induced myocardial infarction in rats.Methods and results: Male Wistar rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with oleuropein at two different doses (20 and 40 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop+Oleu20) and (Isop+Oleu40) groups. The subcutaneous injection of isoproterenol (100 mg/kg body weight) to untreated rats for two consecutive days showed significant increases in ST-segment elevation, heart weight index and alteration in the ECG pattern and hemodynamic function. Else, serum levels of cardiac troponin-T, creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) underwent a notable rise in serum of Isop group by (345, 82, 73 and 106%, respectively) as compared to normal rats. Isoproterenol-induced myocardial injury was evidenced by alteration in serum lipids profile and increased activities of pancreatic lipase by 94% and angiotensin-converting enzyme (ACE) by 78% which reflects the occurrence of cardiac remodeling process. The histopathological findings of the infarcted group showed myocardium necrosis and cells inflammatory infiltration. However, the treatment with oleuropein gave a good protection of the myocardium by decreasing cardiac injury markers specially troponin-T, restoring hemodynamic parameters and attenuating cardiac remodeling process through inhibition of ACE activity.Conclusion: Oleuropein offers high preventive effects from cardiac remodeling process in rats with acute myocardial infarction.