Fluorophore-quencher distance correlation functions from single-molecule photon arrival trajectories.
Fluorophore-quencher distance correlation functions from single-molecule photon arrival trajectories.
复制标题
单分子光子到达轨迹的荧光团-猝灭剂距离相关函数。
DOI：
10.1021/jp045398q
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Szabo,Attila
中科院分区：
文献类型：
--
作者：
Gopich,Irina;Szabo,Attila
We generalize and simplify the method of Yang and Xie (J. Chem.Phys.2002,117, 10965) to obtain distance correlation functions from photon arrival trajectories of single fluorophores whose lifetime, [k(r)]-1, depends on the distance to a quencher. It is assumed that this distance does not change during the fluorescence lifetime. The experimental trajectory is first transformed by replacing the delay time (i.e., the interval between the photon arrival and the nearest laser pulse) by a certain function of this delay time. This function is the inverse Laplace transform ofr(k), which is the solution ofk(r) = 1. The correlation function of the transformed data then directly gives the distance correlation function. Illustrative examples include Förster energy transfer and quenching due to electron transfer.
