Age-dependent Degradation of the Protein Adsorption Capacity of Titanium
Age-dependent Degradation of the Protein Adsorption Capacity of Titanium
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DOI：
10.1177/0022034509339567
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发表时间：
2009-07-01
影响因子：
7.6
通讯作者：
Ogawa, T.
中科院分区：
文献类型：
--
作者：
Hori, N.;Att, W.;Ogawa, T.
报告的骨-种植体接触百分比远低于理想的100%。我们测试了一个假设，即钛的蛋白质吸附能力，这是骨整合过程中的关键，随着时间的推移，在其使用前发生变化。制备机加工、酸蚀刻和喷砂表面，并在黑暗环境条件下储存3天、1周或4周。对于所有的表面，蛋白质吸附随着储存时间的增加而减少，其减少率取决于钛的地形。4周后，由酸蚀刻表面吸附的白蛋白和纤连蛋白的量分别仅为孵育2小时后由新鲜表面吸附的量的20%和35%，并且即使在24小时后也保持相当低的量。钛的蛋白质吸附的这种时间依赖性降解与其自然降低的亲水性相关，这在镍和铬表面没有观察到，表明钛特异性生物老化。
Reported bone-implant contact percentages are far below the ideal 100%. We tested a hypothesis that the protein adsorption capability of titanium, which is critical to the process of osseointegration, changes over time before its use. Machined, acid-etched, and sandblasted surfaces were prepared and stored under dark ambient conditions for 3 days, 1 week, or 4 weeks. For all surfaces, protein adsorption decreased as the storage time increased, and their decreasing rates were dependent on titanium topography. After 4 weeks, the amounts of albumin and fibronectin adsorbed by the acid-etched surface were only 20% and 35%, respectively, of that adsorbed by the fresh surface after 2 hours of incubation, and remained substantially low even after 24 hours. This time-dependent degradation in protein adsorption of titanium correlated with its naturally decreasing hydrophilicity, which was not observed for the nickel and chromium surfaces, indicating a titanium-specific biological aging.