A Rapid, Direct, Quantitative, and Label‐Free Detector of Cardiac Biomarker Troponin T Using Near‐Infrared Fluorescent Single‐Walled Carbon Nanotube Sensors
A Rapid, Direct, Quantitative, and Label‐Free Detector of Cardiac Biomarker Troponin T Using Near‐Infrared Fluorescent Single‐Walled Carbon Nanotube Sensors
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DOI：
10.1002/adhm.201300033
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发表时间：
2014-03
影响因子：
10
通讯作者：
Jingqing Zhang;S. Kruss;A. Hilmer;S. Shimizu;Zeke Schmois;Flor De La Cruz;P. Barone;N. Reuel;D. Heller;M. Strano
中科院分区：
文献类型：
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作者：
Jingqing Zhang;S. Kruss;A. Hilmer;S. Shimizu;Zeke Schmois;Flor De La Cruz;P. Barone;N. Reuel;D. Heller;M. Strano
根据美国疾病控制与预防中心 (2013) 的数据，胸痛患者占美国急诊室就诊人数的 10%。为了对这些患者进行分类，心脏生物标志物肌钙蛋白 I 和 T 被认可为急性心肌梗塞（AMI 或心脏病发作）的标准指标。因此，开发一种用于肌钙蛋白检测的快速护理点 (POC) 设备引起了人们的极大兴趣。在这项工作中，展示了一种使用荧光单壁碳纳米管 (SWCNT) 进行快速、定量、无标记检测的方法，该方法专门用于心肌肌钙蛋白 T (cTnT) 检测。壳聚糖包裹的碳纳米管交联形成薄凝胶，并用次氮基三乙酸 (NTA) 部分进一步功能化。 Ni2+ 螯合后，Ni2+-NTA 基团与六组氨酸修饰的肌钙蛋白抗体结合，该抗体特异性识别目标蛋白肌钙蛋白 T。当肌钙蛋白 T 与抗体结合时，传感器的局部环境发生变化，从而可以通过 SWCNT 带隙荧光的强度变化直接检测肌钙蛋白。该平台代表了第一个用于 cTnT 检测的近红外 SWCNT 传感器阵列。检测可在 5 分钟内完成，表现出对 cTnT 浓度的线性响应和 100 ng mL−1 (2.5 nm) 的实验检测限。该平台为未来的 POC AMI 检测提供了一个有前景的新工具。此外，该工作提出了两种分别量化碳水凝胶基质中胺和羧基数量的新方法。
Patients with chest pain account for 10% of US emergency room visits according to data from the Center for Disease Control and Prevention (2013). For triage of these patients, cardiac biomarkers troponin I and T are endorsed as standard indicators for acute myocardial infarction (AMI, or heart attack). Thus, there is significant interest in developing a rapid, point‐of‐care (POC) device for troponin detection. In this work, a rapid, quantitative, and label‐free assay, which is specific for cardiac troponin T (cTnT) detection, using fluorescent single‐walled carbon nanotubes (SWCNTs), is demonstrated. Chitosan‐wrapped carbon nanotubes are cross‐linked to form a thin gel that is further functionalized with nitrilotriacetic acid (NTA) moieties. Upon chelation of Ni2+, the Ni2+‐NTA group binds to a hexa‐histidine‐modified troponin antibody, which specifically recognizes the target protein, troponin T. As the troponin T binds to the antibody, the local environment of the sensor changes, allowing direct troponin detection through intensity changes in SWCNT bandgap fluorescence. This platform represents the first near‐infrared SWCNT sensor array for cTnT detection. Detection can be completed within 5 min, demonstrating a linear response to cTnT concentration and an experimental detection limit of 100 ng mL−1 (2.5 nm). This platform provides a promising new tool for POC AMI detection in the future. Moreover, the work presents two new methods of quantifying the number of amines and carboxylic groups, respectively, in a carbon hydrogel matrices.