Immunological comparison of pregnant Dahl salt-sensitive and Sprague-Dawley rats commonly used to model characteristics of preeclampsia.
Immunological comparison of pregnant Dahl salt-sensitive and Sprague-Dawley rats commonly used to model characteristics of preeclampsia.
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妊娠 Dahl 盐敏感大鼠和常用于模拟先兆子痫特征的 Sprague-Dawley 大鼠的免疫学比较。
DOI：
10.1152/ajpregu.00298.2020
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sasser,JenniferM
中科院分区：
文献类型：
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作者：
Taylor,ErinB;George,EricM;Ryan,MichaelJ;Garrett,MichaelR;Sasser,JenniferM
妊娠Dahl盐敏感（S）大鼠是一种已建立的叠加自发性先兆子痫临床前模型，其特征为高血压加重、尿蛋白排泄增加和胎儿死亡增加。由于人类先兆子痫妊娠中存在潜在的免疫系统功能障碍，我们假设怀孕的Dahl S大鼠也会改变免疫状态。在Dahl S模型中评估妊娠晚期期间的免疫系统活化，并与经受假手术或降低子宫灌注压（RUPP）的手术的健康妊娠Sprague-Dawley（SD）大鼠进行比较。循环免疫球蛋白和细胞因子水平分别通过酶联免疫吸附试验（ELISA）和Milliplex磁珠试验进行测定，并使用流式细胞术测定循环、脾脏和胎盘免疫细胞的百分比。与未孕Dahl S大鼠相比，妊娠Dahl S大鼠脾脏中的CD 4 +T细胞（特别是TNFα+ CD 4 +T细胞）增加。与Dahl处女大鼠相比，Dahl大鼠外周血中的中性粒细胞也增加，B细胞减少。接受RUPP程序的SD大鼠循环单核细胞增加，IFN-γ + CD 4+脾T细胞增加。总之，这些发现表明，T细胞活性失调是一个重要因素，在怀孕的达尔S大鼠和SD大鼠后，RUPP程序。
The pregnant Dahl salt-sensitive (S) rat is an established preclinical model of superimposed spontaneous preeclampsia characterized by exacerbated hypertension, increased urinary protein excretion, and increased fetal demise. Because of the underlying immune system dysfunction present in preeclamptic pregnancies in humans, we hypothesized that the pregnant Dahl S rat would also have an altered immune status. Immune system activation was assessed during late pregnancy in the Dahl S model and compared with healthy pregnant Sprague-Dawley (SD) rats subjected to either a sham procedure or a procedure to reduce uterine perfusion pressure (RUPP). Circulating immunoglobulin and cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA) and Milliplex bead assay, respectively, and percentages of circulating, splenic, and placental immune cells were determined using flow cytometry. The pregnant Dahl S rat exhibited an increase in CD4+T cells, and specifically TNFα+CD4+T cells, in the spleen compared with virgin Dahl S rats. The Dahl also had increased neutrophils and decreased B cells in the peripheral blood as compared with Dahl virgin rats. SD rats that received the RUPP procedure had increases in circulating monocytes and increased IFN-ɣ+CD4+splenic T cells. Together these findings suggest that dysregulated T cell activity is an important factor in both the pregnant Dahl S rats and SD rats after the RUPP procedure.