GAMMA-GLUTAMYL-TRANSFERASE ACTIVITY IN HUMAN PLATELETS - QUANTIFICATION OF ACTIVITY, ISOENZYME CHARACTERIZATION AND POTENTIAL CLINICAL RELEVANCE
GAMMA-GLUTAMYL-TRANSFERASE ACTIVITY IN HUMAN PLATELETS - QUANTIFICATION OF ACTIVITY, ISOENZYME CHARACTERIZATION AND POTENTIAL CLINICAL RELEVANCE
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DOI：
10.3109/09537109509078455
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发表时间：
1995-08-01
期刊：
影响因子：
3.3
通讯作者：
MIKHAILIDIS, DP
中科院分区：
文献类型：
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作者：
GURDOL, F;NWOSE, OM;MIKHAILIDIS, DP
13名健康、不吸烟的女性志愿者的血小板膜糖蛋白中谷氨酰转氨酶活性为13.6u/g蛋白质，男性为20.3(10.1-26.0)，差异有统计学意义(p=0.034)。血小板与血清GGT活性显著相关(p<；0.04)。血小板似乎只含有GGT4同工酶，这种酶的部分活性是以聚集体的形式或与膜/蛋白质相连的。这种活性是由Triton X-100和胰酶释放并随GGT 4迁移的，常规测定的血清GGT活性会受到血小板释放的GGT的影响，因此，临床上(如心肌梗死、糖尿病、外周血管疾病)合并血小板功能亢进时血清GGT活性可能升高，血小板GGT可能影响细胞内一氧化氮供体S-亚硝基-谷胱甘肽的水平，血清GGT活性与血小板功能指标之间的潜在相关性值得研究。
Gamma-glutamyltransferase (GGT) activity in human platelet sonicates was 13.6 u/g of protein (range: 7.9-25.0) in 13 healthy, non-smoking, female volunteers; corresponding values in 16 males were: 20.3 (10.1-26.0), These values differed significantly (p = 0.034). Platelet and serum GGT activity correlated significantly (p < 0.04). Platelets seem to contain only the isoenzyme GGT 4, Part of this enzyme activity is in the form of aggregates or linked with membranes/proteins. This activity is released by Triton X-100 and trypsin and migrates as GGT 4, Serum GGT activity, a measurement in routine use, could be influenced by GGT released by platelets, It is therefore of interest that serum GGT activity can be increased in clinical conditions (e,g, myocardial infarction, diabetes, peripheral vascular disease) associated with platelet hyperactivity, Platelet GGT may influence intracellular S-nitrosoglutathione (a putative nitric oxide donor) levels, Potential associations between serum GGT activity and platelet function indices deserve investigation.