Rohon-Beard neuron origin from blastomeres of the 16-cell frog embryo
Rohon-Beard neuron origin from blastomeres of the 16-cell frog embryo
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Rohon-Beard 神经元起源于 16 细胞青蛙胚胎的卵裂球
作者：
M. Jacobson
通过在16细胞期将山葵过氧化物酶注射到单个卵裂球中，然后对标记和未标记的罗洪-比尔德神经元进行计数，定量确定非洲爪蟾罗洪-比尔德神经元的克隆来源。选择了两种不同的解理模式。在模式X中，所有的Rohon-Beard神经元来源于每侧的三个卵裂球（V1.1、V1.2和V2.2）；在模式Y中，所有Rohon Beard神经元均来源于两侧的两个卵裂球（V1.2和V2.2）。幼虫期32 ~ 34的Rohon-Beard神经元计数显示96% ~ 100%（平均99%）来自同侧卵裂球；其中，68 - 90%（平均75%）来自V1.2, 20 - 31%（平均24%）来自V2.2, 0 - 7%来自V1.1。讨论了罗洪-比尔德神经元区域限制起源的意义。
Clonal origins of Rohon-Beard neurons in Xenopus were determined quantitatively by injecting horseradish peroxidase into individual blastomeres at the 16-cell stage and later counting labeled and unlabeled Rohon-Beard neurons. Two different patterns of cleavage were selected. In pattern X, all Rohon-Beard neurons originated from three blastomeres (V1.1, V1.2, and V2.2) on each side; in pattern Y, all Rohon Beard neurons originated from two blastomeres (V1.2 and V2.2) on each side. Counts of Rohon-Beard neurons at larval stages 32 to 34 showed that 96 to 100% (mean 99%) originated from blastomeres on the same side; of these, 68 to 90% (mean 75%) descended from V1.2, 20 to 31% (mean 24%) descended from V2.2, 0 to 7% descended from V1.1. The significance of the regionally restricted origin of Rohon-Beard neurons is discussed.