Isolation and characterization of rice lesion-mimic mutants with enhanced resistance to rice blast and bacterial blight
Isolation and characterization of rice lesion-mimic mutants with enhanced resistance to rice blast and bacterial blight
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DOI：
10.1016/s0168-9452(02)00134-6
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发表时间：
2002-08-01
期刊：
影响因子：
5.2
通讯作者：
Okamoto, M
中科院分区：
文献类型：
--
作者：
Mizobuchi, R;Hirabayashi, H;Okamoto, M
我们从13 000M水稻品系中分离到5个感染稻瘟病菌后症状减轻的拟斑突变体。遗传分析表明，1个突变体与Spl5等位，命名为Spl5-2；另外4个突变体来自新的基因座，分别命名为Spl12、Spl13、Spl14和Spl15。Spl12和Spl15为显性突变，其余为隐性突变。显性突变体(Spl12和Spl15)在早期发育即可见病斑，而隐性突变体(Spl5-2、Spl13和Spl14)在播种后8~10周即可见病斑。利用来自14个基因座的SPL突变体对稻瘟病和白叶枯病的抗性进行了分析。在Spl4、Spl5-1、Spl5-2、Spl7、Spl10、Spl12、Spl13、Spl14和Spl15植株中诱导了对两个稻瘟病菌强毒株的抗性。此外，白叶枯病病原菌野油菜黄单胞菌(Xanthomonas Campestris)的生长。在这9个突变体中，水稻也受到抑制。这些结果表明，许多SPL基因增强了一般的抗病能力。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
We isolated five lesion-mimic mutants that showed reduced symptoms after infection with the rice blast fungus Magnaporthe grisea from 13 000 M, lines of rice (Oryza sativa L.). Genetic analysis showed that one mutant was allelic to spl5 and this was named spl5-2; the other four were derived from new loci and named Spl12, spl13, spl14, and Spl15. Spl12 and Spl15 were dominant mutations, while the others were recessive. Lesions were visible in the dominant mutants (Spl12 and Spl15) from an early developmental stage, while lesions were visible in the recessive mutations (spl5-2, spl13, and spl14) from 8-10 weeks after sowing. We analyzed the resistance to rice blast and bacterial blight using spl mutants derived from 14 loci. Resistance to two virulent strains of M. grisea was induced in spl4, spl5-1, spl5-2, spl7, spl10, Spl12, spl13, spl14, and Spl15 plants. Moreover, growth of the bacterial blight pathogen Xanthomonas campestris pv. oryzae was also suppressed in these nine mutants. These results demonstrate that many of the SPL genes enhance general disease resistance. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.