Genomic insights into the lifestyles, functional capacities and oleagenicity of members of the fungal family Trichosporonaceae
Genomic insights into the lifestyles, functional capacities and oleagenicity of members of the fungal family Trichosporonaceae
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DOI：
10.1038/s41598-020-59672-2
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发表时间：
2020-02-17
影响因子：
4.6
通讯作者：
Ochsenreither, Katrin
中科院分区：
文献类型：
--
作者：
Aliyu, Habibu;Gorte, Olga;Ochsenreither, Katrin
丝孢酵母科包含六个生理和生态上不同的真菌属，包括人类致病菌类群以及生物技术感兴趣的酵母菌，特别是那些积累大量单细胞油（SCOs）的产油类群。在这里，我们对该家族的33名成员进行了比较基因组分析，以期深入了解其生活方式和利基专业化的分子决定因素。系统基因组分析显示，可能会影响后续分析的三个菌株的错误鉴定。预测的蛋白质编码序列的评估表明，自由生活的家庭成员港更多的碳水化合物活性酶（CAZYmes），金属和丝氨酸肽酶相比，其主机相关的同行。所研究菌株的基因组中编码的选定脂质生物合成酶的系统发育揭示了与核心基因组同源性不一致的一些蛋白质的不同进化历史。然而，记录的产油成员明显聚类的基础上的乙酰辅酶A羧化酶（ACC），ATP-柠檬酸合酶（ACS）和异柠檬酸脱氢酶[NADP]（ICDH），这是在这些酵母的脂质生物合成途径中的主要蛋白质的基因的上游调控区的构成，这表明在这些基因的调控可能的模式。
Trichosporonaceae incorporates six genera of physiologically and ecologically diverse fungi including both human pathogenic taxa as well as yeasts of biotechnological interest, especially those oleagenic taxa that accumulate large amounts of single cell oils (SCOs). Here, we have undertaken comparative genomic analysis of thirty-three members of the family with a view to gain insight into the molecular determinants underlying their lifestyles and niche specializations. Phylogenomic analysis revealed potential misidentification of three strains which could impact subsequent analyses. Evaluation of the predicted proteins coding sequences showed that the free-living members of the family harbour greater numbers of carbohydrate active enzymes (CAZYmes), metallo- and serine peptidases compared to their host-associated counterparts. Phylogenies of selected lipid biosynthetic enzymes encoded in the genomes of the studied strains revealed disparate evolutionary histories for some proteins inconsistent with the core genome phylogeny. However, the documented oleagenic members distinctly cluster based on the constitution of the upstream regulatory regions of genes encoding acetyl-CoA carboxylase (ACC), ATP-citrate synthase (ACS) and isocitrate dehydrogenase [NADP] (ICDH), which are among the major proteins in the lipid biosynthetic pathway of these yeasts, suggesting a possible pattern in the regulation of these genes.