Evolution of dengue virus type 2 during two consecutive outbreaks with an increase in severity in southern Taiwan in 2001-2002
Evolution of dengue virus type 2 during two consecutive outbreaks with an increase in severity in southern Taiwan in 2001-2002
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DOI：
10.4269/ajtmh.2008.79.495
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发表时间：
2008-10-01
影响因子：
3.3
通讯作者：
Wang, Wei-Kung
中科院分区：
文献类型：
--
作者：
Chen, Hui-Ling;Lin, Su-Ru;Wang, Wei-Kung
为了研究病毒决定因素和进化与疫情严重程度的关系，我们在2001年和2002年台湾南部登革热疫情期间，连续检测了31例患者（14例登革热，17例登革出血热，DHF）的血浆DENV-2 （DENV-2）序列，其中登革出血热病例总数和病例比例均有所增加。对两次暴发的病毒包膜(E)和全基因组序列进行分析，发现E、NS1、NS4A和NS5基因有5个核苷酸变化。没有一个与1997年古巴DENV-2暴发中报告的相同，这表明与严重暴发相关的病毒决定因素依赖于基因型。与以往关于谱系转换间隔年的报道相比，我们关于2002年病毒在不到6个月的时间内由2001年病毒的一个小变种进化而来的发现是新颖的，并且可能代表了DENV从一次爆发到另一次爆发的进化机制。
To investigate viral determinants and evolution linked to outbreak with increased severity, we examined dengue virus type 2 (DENV-2) sequences from plasma of 31 patients (14 dengue fever, 17 dengue hemorrhagic fever, DHF) continuously during the 2001 and 2002 outbreaks in southern Taiwan, in which both the total cases and proportion of DHF cases increased. Analysis of envelope (E) and full-genome sequences between viruses of the two outbreaks revealed 5 nucleotide changes in E, NS1, NS4A, and NS5 genes. None was identical to those reported in the DENV-2 outbreak in Cuba in 1997, suggesting viral determinants linked to severe outbreak are genotype dependent. Compared with previous reports of lineage turnover years apart, our findings that the 2002 viruses descended from a minor variant of the 2001 viruses in less than 6 months was novel, and may represent a mechanism of evolution of DENV from one outbreak to another.