A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II.
A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialylated lacto-N-fucopentaose II.
复制标题
DOI：
10.1016/s0021-9258(19)45389-1
复制
发表时间：
1982-12
期刊：
影响因子：
--
通讯作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
中科院分区：
文献类型：
--
作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
从用结肠直肠癌细胞系免疫的小鼠获得的杂交瘤产生的两种单克隆抗体特异性结合人胃肠癌细胞。这种抗体在癌细胞系中的抗原，一种单唾液酸神经节苷脂，已经被分离出来。它的碳水化合物结构可能是（公式：见正文）。该寡糖是乳糖-N-岩藻五糖II（人莱亚血型抗原的半抗原）的唾液酸衍生物。从1g湿重的细胞中获得约30微克神经节苷脂。神经节苷脂的放射自显影检测在一些癌和胎粪的脂质提取物，但不是在正常成人组织的脂质提取物。用固相放射免疫法检测了21例结肠腺癌中12例、5例胃腺癌中4例和7例胰腺癌中4例的脂质提取物中的抗原。在5例食管癌或正常结肠和胃粘膜、胰腺、肾脏、肝脏和骨髓的脂质提取物中未检测到抗原。
Two monoclonal antibodies produced by hybridomas obtained from a mouse immunized with a colorectal carcinoma cell line bind specifically to human gastrointestinal cancer cells. The antigen of this antibody in the carcinoma cell line, a monosialoganglioside, has been isolated. Its carbohydrate structure is probably (formula: see text). This oligosaccharide is a sialyl derivative of lacto-N-fucopentaose II, a hapten of the human Lea blood group antigen. About 30 micrograms of ganglioside is obtained from 1 g of cells, wet weight. The ganglioside was detected by autoradiography in lipid extracts of some carcinomas and of meconium, but not in lipid extracts of normal adult tissues. Antigen was detected by solid phase radioimmunoassay in lipid extracts from 12 out of 21 adenocarcinomas of the colon, from 4 out of 5 gastric adenocarcinomas, and from 4 out of 7 pancreatic carcinomas. Antigen was not detected in lipid extracts from 5 esophageal carcinomas or from normal colon and gastric mucosa, pancreas, kidney, liver, and bone marrow.