Calcium phosphate/poly(D,L-lactic-co-glycolic acid) composite bone substitute materials: evaluation of temporal degradation and bone ingrowth in a rat critical-sized cranial defect.
Calcium phosphate/poly(D,L-lactic-co-glycolic acid) composite bone substitute materials: evaluation of temporal degradation and bone ingrowth in a rat critical-sized cranial defect.
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DOI：
10.1111/j.1600-0501.2011.02218.x
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发表时间：
2012-02
影响因子：
4.3
通讯作者：
F. C. J. van de Watering;J. J. van den Beucken-J.;X. Walboomers;John A. Jansen
中科院分区：
文献类型：
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作者：
F. C. J. van de Watering;J. J. van den Beucken-J.;X. Walboomers;John A. Jansen
本研究旨在提供在大鼠临界尺寸颅骨缺损中使用复合（C）骨缺损填充材料（由磷酸钙骨水泥（CaP）和聚（D，L-乳酸-共-乙醇酸）（PLGA）微粒（20或30重量%）组成）的材料降解和骨形成的时间信息。材料与方法在48只大鼠中制造临界尺寸的双皮质颅骨缺损，并将CaP/PLGA骨水泥复合物植入4、8和12周（n=8）。结果对取出的样本进行组织学分析，结果显示，与C20%（12周时剩余植入物高达94.8 ± 2.1%）相比，C30%（12周时剩余植入物高达89.4 ± 4.4%）的植入物降解明显更快，尽管总体降解有限。尽管两个实验组的骨形成有限（C20%高达685765.9 μm（2）vs. C30%为917603.3 μm（2）），但C30%显示总骨形成的时间显著增加。在所有植入期间，C20%和C30%的缺陷桥接百分比相当（C20%的范围为第4周的40 ± 25.5%至第12周的65 ± 20%; C30%的范围为第4周的51.8 ± 7.8%至第12周的70.5 ± 16.2%）。结论CaP/PLGA骨水泥复合材料中PLGA微粒的加入加速了材料的降解，但不影响骨形成。有必要进一步优化复合材料，以增加对降解和组织向内生长的控制。
OBJECTIVES The present study aimed to provide temporal information on material degradation and bone formation using composite (C) bone defect filler materials consisting of calcium phosphate cement (CaP) and poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles (20 or 30 wt%) in rat critical-sized cranial defects. MATERIALS AND METHODS Critical-sized bicortical cranial defects were created in 48 rats and CaP/PLGA cement composites were implanted for 4, 8 and 12 weeks (n=8). RESULTS Histological analysis of the retrieved specimens revealed that implant degradation was significantly faster for C30% (remaining implant up to 89.4 ± 4.4% at 12 weeks) compared with C20% (remaining implant upto 94.8 ± 2.1% at 12 weeks), albeit that overall degradation was limited. Although bone formation was limited in both experimental groups (upto 685765.9 μm(2) for C20% vs. 917603.3 μm(2) for C30%), C30% showed a significant temporal increase of total bone formation. The percentage of defect bridging was comparable for C20% and C30% at all implantation periods (range 40 ± 25.5% at week 4 to 65 ± 20% at week 12 for C20%; range 51.8 ± 7.8% at week 4 to 70.5 ± 16.2% at week 12 for C30%). CONCLUSION The amount of PLGA-microparticles in the CaP/PLGA cement composites demonstrated acceleration of material degradation, while bone formation was found not to be influenced. Further optimization of the composite material is necessary to increase control over degradation and tissue ingrowth.