1.5 MHz ultrasound irradiation of human lymphocytes.
1.5 MHz ultrasound irradiation of human lymphocytes.
复制标题
1.5 MHz 超声波照射人体淋巴细胞。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
S. Leeman
中科院分区：
文献类型：
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作者：
M. Hedges;S. Leeman
将人淋巴细胞培养物暴露于1.5 MHz连续波超声，并且证明了细胞死亡，如通过固缩监测的，在通常治疗范围内的强度（小于1.7 W/cm 2，空间平均值）的超声处理后立即发生。受影响的细胞数量仅由超声强度决定，但它们通过固缩周期进行的速率由强度和暴露持续时间改变。有一个明确的指示强度阈值的影响约1.1 W/cm 2。脉冲1.5 MHz超声（70 μ s，1：1脉冲，1.7 W/cm 2时空平均值）导致对超声处理的可测量响应延迟15-20小时。结果表明，细胞内存在的溶酶体酶抑制剂强烈修改的过程中的超声作用。有证据表明，基本的相互作用机制是通过空化过程，但也有一些困难，这种解释，这也进行了讨论。
Human lymphocyte cultures are exposed to 1.5 MHz continuous wave ultrasound, and it is demonstrated that cell death, as monitored by pyknosis, follows immediately on sonication at intensities within the usual therapeutic range (less than 1.7 W/cm2,spatial average). The number of cells affected is determined by the ultrasound intensity only, but the rate at which they proceed through their pyknosis cycle is modified by both the intensity and the duration of exposure. There is a clear indication of an intensity threshold for the effect approximately 1.1 W/cm2. Pulsed 1.5 MHz ultrasound (70 mus, 1 :1 pulses, 1.7 W/cm2 space-time average) results in a 15-20 hour delay in the measurable response to sonication. It is shown that the intracellular presence of a lysosomal-enzyme inhibitor strongly modifies the course of the ultrasound action. Evidence is presented to suggest that the basic interaction mechanism is via a cavitation process, but there are some difficulties with this interpretation, which are also discussed.