Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets
Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets
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DOI：
10.3382/ps.2009-00291
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发表时间：
2009-12-01
期刊：
影响因子：
4.4
通讯作者：
Kitazawa, H.
中科院分区：
文献类型：
--
作者：
Sato, K.;Takahashi, K.;Kitazawa, H.
测定了饲喂简氏乳杆菌TL2937-、加色乳杆菌JCM1131(T)-、德氏乳杆菌SSP免疫活性乳酸菌的肉仔鸡断奶后第1周肠道免疫活性细胞的发育变化。保加利亚乳杆菌NIAIB6或巴西乳杆菌TL2919添加日粮。饲喂免疫生物饲料的雏鸡，除3日龄脾重、1日龄法氏囊重和1日龄法氏囊重外，其余均略高于对照组(P<0.05)。体重和肝脏重量在不同处理间没有显著差异。在3日龄和7日龄分别饲喂添加简氏乳杆菌TL2937和加色乳杆菌TL2919的雏鸡，前肠T细胞相关基因[分化簇3(CD3)、白介素2(IL-2)和干扰素-γ(干扰素-γ)]的表达显著高于对照组。Toll样受体(Toll-like Receptor，TLR)mRNA在饲喂免疫生物饲料的雏鸡前肠中的表达水平有增加的趋势，但Delbreckii SSP除外。Bulgaricus NIAIB6，与对照雏鸡的表达水平进行比较。添加免疫活性乳酸菌不影响法氏囊Bu-1mRNA的表达水平。这些结果表明，免疫生物剂，特别是加瑟氏乳杆菌TL2919，可能作为免疫调节剂来刺激初生雏鸡的肠道相关免疫系统，从而在不降低生长性能的情况下保护它们免受疾病的影响，作为抗生素的可能替代品。
Developmental changes in immunocompetent cells of the gut during the first week posthatch were determined in broiler chicks fed immunobiotic lactic acid bacteria in the form of Lactobacillus jensenii TL2937-, Lactobacillus gasseri JCM1131(T)-, Lactobacillus delbrueckii ssp. bulgaricus NIAIB6-, or L. gasseri TL2919-supplemented diets. The relative weights of spleen and bursa of Fabricius in chicks fed the immunobiotic diets were slightly higher than the control valued at 1 and 3 d of age, with the exception of spleen weight in the L. gasseri JCM1131(T) at 3 d of age, the bursa of Fabricius weight in the L. gasseri JCM1131(T) at 1 and 3 d of age, and bursa of Fabricius weight in the L. gasseri TL2919 group at 1 d of age. There were no significant differences in body and liver weights among the treatments. When chicks were fed the L. jensenii TL2937- or L. gasseri TL2919-supplemented diets, expression of T cell-related mRNA [cluster of differentiation 3 (CD3), interleukin-2 (IL-2), and interferon-gamma (IFN-gamma)] in the foregut was significantly higher than that of control chicks at 3 or 7 d of age. Expression levels of toll-like receptor (TLR) mRNA tended to increase in the foregut of chicks fed the immunobiotic diets, except for the L. delbrueckii ssp. bulgaricus NIAIB6, compared with expression levels in control chicks. The Bu-1 mRNA expression levels in the bursa of Fabricius were not affected by the supplementations with immunobiotic lactic acid bacteria. These results show that immunobiotics, particularly L. gasseri TL2919, might be useful as immunomodulators to stimulate the gut-associated immune system in neonatal chicks, and thereby protect them from disease without decreasing growth performance as a possible substitution of antibiotics.