Directed and Modular Protein Immobilization on Gold and Silver Nanoparticles.
Directed and Modular Protein Immobilization on Gold and Silver Nanoparticles.
复制标题
金和银纳米粒子上的定向和模块化蛋白质固定化。
DOI：
10.1007/978-1-0716-0319-2_17
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Saccardo A
中科院分区：
文献类型：
--
作者：
Saccardo A
蛋白质与金纳米颗粒 (AuNP)、银纳米颗粒 (AgNP) 或其他金属纳米颗粒 (NP) 的缀合通常可以使用被动吸附来实现。尽管这种方法简单有效，但由于与金属表面紧密接触，通常无法控制蛋白质的方向和变性。这里描述的方法利用接头蛋白，该接头蛋白能够以定向且稳定的方式吸附到 NP 表面，同时能够直接附着到其他感兴趣的蛋白质。
Conjugation of proteins to gold nanoparticles (AuNP), silver nanoparticles (AgNP), or other metal nanoparticles (NPs) can often be achieved using passive adsorption. Although such an approach is simple and effective, there is usually no control over the orientation of the protein and denaturation due to close contact with the metal surface. The method described here makes use of adapter proteins which have the ability to adsorb to the NP surface in an oriented and stable way and at the same time enable straightforward attachment to other proteins of interest.
用重组蛋白装饰纳米粒子的即插即用方法。
影响因子：
5.5
作者：
Enrico Ferrari
通讯作者：
Enrico Ferrari