Immunological responses to recombinant cysteine synthase A of Brucella abortus in BALB/c mice
Immunological responses to recombinant cysteine synthase A of Brucella abortus in BALB/c mice
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DOI：
10.1007/s11274-012-1247-3
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发表时间：
2013-05-01
影响因子：
4.1
通讯作者：
Kumar, Subodh
中科院分区：
文献类型：
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作者：
Jain, Shikha;Afley, Prachiti;Kumar, Subodh
布鲁氏菌病是一种世界性的人畜共患病。没有布鲁氏菌疫苗可用于人类，现有的动物疫苗也有局限性。需要开发一种安全有效的预防人和动物布鲁氏菌病的疫苗。在本研究中，我们在大肠杆菌中产生重组布鲁氏菌半胱氨酸合酶A（rCysK），并通过金属亲和层析将其纯化至均一。用免疫佐剂、氢氧化铝凝胶和不加佐剂的BALB/c小鼠评价rCysK的免疫原性和免疫保护效果。所有免疫小鼠均观察到以IgG 1为主的高滴度抗rCysK IgG抗体。用rCysK刺激后，用氢氧化铝凝胶配制的CysK免疫的小鼠的脾淋巴细胞产生显著水平的IFN-γ。对强毒B攻毒的保护。在BALB/c小鼠中测定了流产病毒544株，并且当与PBS对照相比时，在所有CysK免疫组中观察到显著的保护作用。在所有CysK疫苗组中，在用氢氧化铝凝胶免疫的小鼠中观察到相对较好的保护（1.064单位的保护）。总的来说，研究结果表明，rCysK主要诱导Th 2型免疫应答，并提供针对B的部分保护。流产攻毒。
Brucellosis is a worldwide zoonotic disease. No Brucella vaccine is available for use in humans, and existing animal vaccines have limitations. There is a need to develop a safe and effective vaccine against human and animal brucellosis. In the present study, we generated recombinant cysteine synthase A (rCysK) of Brucella abortus in Escherichia coli and purified it up to homogeneity by metal affinity chromatography. The immunogenicity and protective efficacy of purified rCysK were evaluated in BALB/c mice with Freund's adjuvant, aluminium hydroxide gel or without any adjuvant. High titres of anti-rCysK IgG antibody predominated by IgG1 were observed in all immunized mice. After stimulation with rCysK, the spleen lymphocytes of mice immunized with CysK formulated with aluminium hydroxide gel produced significant levels of IFN-gamma. Protection against challenge with virulent B. abortus strain 544 was determined in BALB/c mice, and significant protection was observed in all CysK immunized groups when compared with PBS control. Among all the CysK vaccine groups, comparatively better protection was observed in mice immunized with aluminium hydroxide gel (1.064 units of protection). Overall, the results of the study suggest that rCysK induces primarily Th2 type of immune response and provides partial protection against B. abortus challenge.