TRIPLE GENE BLOCK PROTEINS OF WHITE CLOVER MOSAIC POTEXVIRUS ARE REQUIRED FOR TRANSPORT
TRIPLE GENE BLOCK PROTEINS OF WHITE CLOVER MOSAIC POTEXVIRUS ARE REQUIRED FOR TRANSPORT
复制标题
DOI：
10.1016/0042-6822(91)90998-q
复制
发表时间：
1991-08-01
期刊：
影响因子：
3.7
通讯作者：
FORSTER, RLS
中科院分区：
文献类型：
--
作者：
BECK, DL;GUILFORD, PJ;FORSTER, RLS
对白三叶花叶痘病毒(WCIMV)三个重叠基因块(三重基因块)编码的蛋白产物的功能进行了测定。突变被引入到每一个三重基因块的开放阅读框中，以及在植物和原生质体中分析的体外RNA转录本中。所有突变体均不能在4个系统寄主和1个局部病斑寄主中诱发症状或扩散，但均能在接种的原生质体中产生后代基因组RNA、亚基因组RNA、外壳蛋白和病毒粒子，表明所有三重基因阻断蛋白都参与了细胞间的传播。根据Potex-、Carla-、Furo-和Hordei病毒群与烟草平纹夜蛾病毒的三重基因块蛋白的同源性，以及WCIMV和大麦条纹花叶病毒三重基因块蛋白的运输功能，这些蛋白被认为是一类新的运输蛋白。
The functions of the protein products encoded by a block of three overlapping genes (the triple gene block) of white clover mosaic potexvirus (WCIMV) have been determined. Mutations were introduced into each of the triple gene block open reading frames andin vitroRNA transcripts assayed in plants and protoplasts. None of the mutants was able to induce symptoms or spread in four systemic hosts and one local lesion host, but all were able to produced progeny genomic RNA, subgenomic RNA, coat protein, and virions in inoculated protoplasts, indicating that all the triple gene block proteins are involved in cell-to-cell spread. Based on observed homologies between the triple gene block proteins of the potex-, carla-, furo-, and hordeivirus groups andNicotiana velutinamosaic virus, and the demonstrated transport function of the WCIMV and barley stripe mosaic virus triple gene block proteins, these proteins are proposed to constitute a new class of transport proteins.