Confocal laser scanning microscopy in study of bone calcification
Confocal laser scanning microscopy in study of bone calcification
复制标题
共聚焦激光扫描显微镜在骨钙化研究中的应用
DOI：
10.1016/j.apsusc.2012.01.163
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发表时间：
2012
影响因子：
6.7
通讯作者：
A. Tanaka
中科院分区：
文献类型：
--
作者：
T. Nishikawa;Mayu Kokubu;Hirohito Kato;K. Imai;A. Tanaka
为了阐明钙化的机制，必须研究拔牙或肿瘤切除后下颌骨和上颌骨的骨再生以及粗糙表面种植体在骨诱导中的应用。对钙化的组织进行化学脱钙或物理研磨，用光学显微镜和透射电子显微镜观察其显微特征，其中显微组织形态发生显著改变。我们研究了共聚焦激光扫描显微镜（CLSM）用于此目的的有用性。将钙黄绿素和茜素红错开给药时间后，用激光共聚焦扫描显微镜观察大鼠牙槽骨和狗股骨移植珊瑚支架或种植体的情况。在大鼠牙槽骨中，以15 μm/d的速度生长，从牙根向近中骨可见新生骨和网状小管钙化。犬股骨移植珊瑚后，可观察到沿着珊瑚间隙有规则的新骨形成。在狗股骨与牙种植体，8周后，新形成的骨进行沿着种植体的粗糙表面。CLSM产生新形成的骨的高放大倍数图像，并且不需要薄切片。
Bone regeneration in mandible and maxillae after extraction of teeth or tumor resection and the use of rough surface implants in bone induction must be investigated to elucidate the mechanism of calcification. The calcified tissues are subjected to chemical decalcification or physical grinding to observe their microscopic features with light microscopy and transmission electron microscopy where the microscopic tissue morphology is significantly altered. We investigated the usefulness of confocal laser scanning microscopy (CLSM) for this purpose. After staggering the time of administration of calcein and alizarin red to experimental rats and dogs, rat alveolar bone and dog femur grafted with coral as scaffold or dental implants were observed with CLSM. In rat alveolar bone, the calcification of newly-formed bone and net-like canaliculi was observed at the mesial bone from the roots progressed at the rate of 15 μm/day. In dog femur grafted with coral, newly-formed bones along the space of coral were observed in an orderly manner. In dog femur with dental implants, after 8 weeks, newly-formed bone proceeded along the rough surface of the implants. CLSM produced high-magnification images of newly-formed bone and thin sections were not needed.
酸蚀植入物的磷酸盐处理对狗模型中植入物附近矿物质沉积率的影响。
DOI：
--
发表时间：
2010
期刊：
The International journal of oral & maxillofacial implants
影响因子：
--
作者：
Foley,ChristineHyon;Kerns,DavidG;Hallmon,WilliamW;Rivera-Hidalgo,Francisco;Nelson,CarlJ;Spears,Robert;Dechow,PaulC;Opperman,LynneA
通讯作者：
Opperman,LynneA
影响因子：
4.3
作者：
Sebaoun JD;Kantarci A;Turner JW;Carvalho RS;Van Dyke TE;Ferguson DJ
通讯作者：
Ferguson DJ