Developing 3D Scaffolds in the Field of Tissue Engineering to Treat Complex Bone Defects
Developing 3D Scaffolds in the Field of Tissue Engineering to Treat Complex Bone Defects
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DOI：
10.1089/3dp.2016.0006
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发表时间：
2016-06-01
影响因子：
3.1
通讯作者：
Soman, Pranav
中科院分区：
文献类型：
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作者：
Albrecht, Lucas D.;Sawyer, Stephen W.;Soman, Pranav
聚合物已被广泛用于开发组织工程领域的3D支架，并且包括某些设计要求，例如生物相容性，结构特性和复杂几何形状内的不同孔隙率，所有这些都具有将活细胞纳入支架结构内的最终目标。在这项工作中，我们提出了使用聚己内酯（PCL）作为基础聚合物的混合线轴的合成和材料表征。我们证明了商业3D熔融沉积成型打印机，如MakerBot，可用于使用三种类型的聚合物线轴打印3D支架：PCL，PCL-聚乳酸和PCL-羟基磷灰石。来自计算机断层扫描的数据可用于使用PCL开发中空多孔融合器。最后，我们证明了原木桩支架能够注入活细胞和明胶水凝胶的混合物，并且在整个打印结构中保持高细胞活力。这项工作可能在治疗复杂骨缺损患者方面有潜在的用途。
Polymers have been extensively used to develop 3D scaffolds in the field of tissue engineering and consist of certain design requirements such as biocompatibility, structural properties, and varying porosity inside of complex geometries, all with the ultimate goal of incorporating living cells within the scaffold structure. In this work, we present the synthesis and material characterization of hybrid spools using polycaprolactone (PCL) as the base polymer. We demonstrate that a commercial 3D Fused Deposition Modeling printer such as MakerBot can be used to print 3D scaffolds using three types of polymer spools: PCL, PCL-poly lactic acid, and PCL-hydroxyapatite. Data derived from computer tomography can be used to develop hollow porous cages using PCL. Finally, we demonstrate that log-pile scaffolds are capable of being infused with a mixture of living cells and gelatin hydrogel and that high cellular viability is maintained throughout the printed structure. This work could be potentially useful in the treatment of patients with complex bone defects.