Prostaglandin D synthase in human megakaryoblastic cells
Prostaglandin D synthase in human megakaryoblastic cells
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DOI：
10.1074/jbc.272.45.28263
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发表时间：
1997-11-07
影响因子：
4.8
通讯作者：
Hayaishi, O
中科院分区：
文献类型：
--
作者：
Mahmud, I;Ueda, N;Hayaishi, O
人血小板的胞质部分不会将前列腺素 (PG) H-2 转化为 PGD(2)。然而，人巨核细胞 CMK 细胞（血小板前体细胞）的匀浆从 PGH(2) 产生 PGD(2)。 PGD合酶活性位于CMK细胞的胞浆中，并且绝对需要谷胱甘肽。催化特性及Western和Northern blotting表明该酶为造血型PGD合酶而非脂质运载蛋白型。当CMK细胞用佛波酯诱导分化为巨核细胞并诱导环氧合酶-1时，PGD合酶活性在2天内增加约2倍，然后下降。在另一种人类巨核细胞系 Dami 中，添加佛波酯后，PGD 合酶增加了约 10 倍。因此，在血小板中检测不到的PGD合酶在巨核细胞分化为巨核细胞的过程中出现。
The cytosol fraction of human platelets did not convert prostaglandin (PG) H-2 to PGD(2). However, a homogenate of human megakaryoblastic CMK cells (precursor cells of platelets) produced PGD(2) from PGH(2). The PGD synthase activity was localized in the cytosol of CMK cells, and absolutely required glutathione. The catalytic properties and Western and Northern blottings indicated that the enzyme was PGD synthase of the hematopoietic type rather than the lipocalin type, When CMK cells were differentiated to megakaryocytes with phorbol ester along with induction of cyclooxygenase-l, the PGD synthase activity increased about 2-fold for 2 days and then decreased. In another human megakaryoblastic cell line, Dami, the PGD synthase increased about 10-fold by the addition of phorbol ester. Thus, the PGD synthase, which was undetectable in platelets, appeared during differentiation of megakaryoblasts to megakaryocytes.