Autoimmune regulator initiates the expression of promiscuous genes in thymic epithelial cells
Autoimmune regulator initiates the expression of promiscuous genes in thymic epithelial cells
复制标题
自身免疫调节剂启动胸腺上皮细胞中混杂基因的表达
DOI：
10.1080/08820130801967841
复制
发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Li, Yi
中科院分区：
文献类型：
--
作者：
Chen, Jibing;Yang, Wei;Li, Yi
外周抗原在胸腺中的表达，被称为混杂基因表达，与T细胞耐受和自身免疫有关。在这里，我们确定胸腺上皮细胞（TECs）是表达多种组织限制性抗原（TRAs）的主要细胞类型。普通自身免疫（非肥胖糖尿病[NOD]）小鼠模型的tec表达的自身免疫调节因子（Aire）和TRAs水平远低于正常tec （Balb/c）。转染Aire质粒导致Balb/c和NOD小鼠培养的tec中TRA表达水平升高；胸腺细胞的存在增强了这种增加。这些数据表明Aire在tec中启动混杂基因表达，并且这种功能可能受到胸腺细胞的控制。
The expression of peripheral antigens in the thymus, known as promiscuous gene expression, has been implicated in T cell tolerance and autoimmunity. Here we identified thymic epithelial cells (TECs) as the main cell type that expresses a diverse range of tissue-restricted antigens (TRAs). The TECs of a common autoimmune (non-obese diabetic [NOD]) mouse model express much lower levels of an autoimmune regulator (Aire) and TRAs than normal (Balb/c) TECs. Transfection of an Aire plasmid led to increased levels of TRA expression in cultured TECs from Balb/c and NOD mice; an increase that was enhanced by the presence of thymocytes. These data show that Aire initiates promiscuous gene expression in TECs, and that this function might be under thymocyte control.