Detection of Salivary IgA Antibodies Against the HlyE Antigen as a Diagnosis of Typhoid Fever
Detection of Salivary IgA Antibodies Against the HlyE Antigen as a Diagnosis of Typhoid Fever
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DOI：
10.7860/jcdr/2016/17801.7909
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发表时间：
2016-06-01
影响因子：
0.2
通讯作者：
Ong, Eugene Boon Beng
中科院分区：
文献类型：
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作者：
Chin, Kai Ling;Redhuan, Nur Eliyana Mohd;Ong, Eugene Boon Beng
简介：伤寒沙门氏菌（S. (yphi)）溶血素E蛋白（HlyE）已被证明是一种通过检测血清中抗HlyE抗体来检测伤寒的敏感和特异性抗原。唾液也是一种有用的诊断液体，因为它也含有对抗细菌病原体的抗体。目的：探讨唾液抗hye抗体检测在伤寒诊断中的应用价值。材料和方法：收集16例在马来西亚圣士大学医院就诊的急性伤寒患者的唾液，这些患者持续发热超过5天，伤寒沙门氏菌血培养阳性。还收集了康复期伤寒患者（n=11）、其他发热患者（n=15）和健康个体（n=25）的唾液。建立了一种ELISA法检测伤寒患者唾液中hye抗体的存在。结果：急性伤寒组平均吸光度值为1.496，高于恢复期伤寒组（0.538）、其他发热组（0.678）和健康人组（0.457）。结论：本研究证明唾液抗hye IgA抗体可作为伤寒诊断的生物标志物。后续研究与更大的样本量将允许优化灵敏度和特异性的分析。这种非侵入性方法可用于大规模筛查项目。
Introduction:The Salmonella typhi (S. (yphi)haemolysin E protein (HlyE) has been shown to be a sensitive and specific antigen for the detection of typhoid fever through the detection of anti-HlyE antibodies in sera. Saliva can also be a useful diagnostic fluid as it also contains antibodies against bacterial pathogens.Aim: This study aims to evaluate the potential detection of salivary anti-HlyE antibodies as a diagnosis of typhoid fever.Materials and Methods: Saliva was collected from acute typhoid patients (n=16) who presented at Hospital Universiti Sains Malaysia with prolonged fever of more than five days and were positive for S. Typhi blood culture. Saliva was also collected from convalescent typhoid patients (n=11), patients with other febrile fevers (n=15), and from healthy individuals (n=25). An ELISA was developed to detect the presence of IgA antibodies against HlyE in the saliva of typhoid patients.Results: The acute typhoid group had a higher mean absorbance value of 1.496 compared to the convalescent typhoid (0.538), other febrile fevers (0.678), and healthy individuals (0.457) group.Conclusion: This study demonstrated the utility of salivary antiHlyE IgA antibody as a biomarker for the diagnosis of typhoid fever. Follow-up studies with a larger sample size will allow the optimization of the sensitivity and specificity of the assay. This non-invasive method can be useful for mass screening programs.