Surface modification of poly(ether urethane urea) with modified dehydroepiandrosterone for improved in vivo biostability.
Surface modification of poly(ether urethane urea) with modified dehydroepiandrosterone for improved in vivo biostability.
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用改性脱氢表雄酮对聚醚聚氨酯脲进行表面改性，以提高体内生物稳定性。
DOI：
10.1002/jbm.a.30271
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Hiltner,Anne
中科院分区：
文献类型：
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作者：
Christenson,ElizabethM;Wiggins,MichaelJ;Anderson,JamesM;Hiltner,Anne
在这项研究中，合成了脱氢表雄酮（DHEA）的脂肪酸氨基甲酸酯衍生物，并将其作为聚氨酯添加剂进行评估，以增加长期生物稳定性。假设该改性降低了DHEA的水溶性，并在植入过程中将添加剂物理锚在聚氨酯中。研究了聚氨酯膜在水中的重量损失作为时间的函数，以确定改性DHEA的聚合物保留。具有未改性的DHEA的聚氨酯膜在第一天具有显著的重量损失（10%），这先前与添加剂的快速浸出相关。具有改性DHEA的聚氨酯膜在所有时间点具有显著更小的重量损失，表明改进的聚合物保留。在Sprague-道利大鼠中皮下植入5周后，检查改性DHEA添加剂对聚醚氨基甲酸酯脲生物稳定性的影响。样品的光学显微照片和红外分析表明，改性DHEA在膜的表面上起霜，形成约10-15微米厚的结晶表面层。在干燥后，该表面层是完整的，如通过衰减全反射-傅里叶变换红外光谱法监测的，表面化学没有可测量的差异。与聚氨酯对照相比，在改性的DHEA表面层下面没有聚氨酯降解的证据。我们得出结论，改性DHEA自组装成保护性表面涂层，可抑制聚氨酯降解。修饰的DHEA表面层的粗糙度阻止了粘附细胞分析以确定添加剂是否保留下调巨噬细胞活性的能力。后续研究将研究表面改性添加剂除了形成不可渗透屏障外，还调节细胞呼吸爆发的能力。这种改善生物稳定性的双峰方法在聚氨酯生物材料领域具有很大的前景。© 2005威利期刊有限公司J Biomed Mater Res 73A：108-115，2005
In this study, a fatty acid urethane derivative of dehydroepiandrosterone (DHEA) was synthesized and evaluated as a polyurethane additive to increase long‐term biostability. The modification was hypothesized to reduce the water solubility of the DHEA and physically anchor the additive in the polyurethane during implantation. Polyurethane film weight loss in water as a function of time was studied to determine the polymer retention of the modified DHEA. The polyurethane film with unmodified DHEA had significant weight loss in the first day (10%) that was previously correlated to rapid leaching of the additive. The polyurethane film with modified DHEA had significantly less weight loss at all time points indicating improved polymer retention. The effect of the modified DHEA additive on the biostability of a poly(ether urethane urea) was examined after 5 weeks of subcutaneous implantation in Sprague‐Dawley rats. Optical micrographs and infrared analysis of the specimens indicated that the modified DHEA bloomed to the surface of the film forming a crystalline surface layer approximately 10–15 microns thick. After explantation, this surface layer was intact without measurable differences in surface chemistry as monitored by attenuated total reflectance‐Fourier transform infrared spectroscopy. There was no evidence of degradation of the polyurethane underneath the modified DHEA surface layer as compared with the polyurethane control. We have concluded that the modified DHEA self‐assembled into a protective surface coating that inhibited degradation of the polyurethane. The roughness of the modified DHEA surface layer prevented adherent cell analysis to determine if the additive retained the ability to down‐regulate macrophage activity. Subsequent studies will investigate the ability of surface‐modifying additives to modulate cellular respiratory bursts in addition to the formation of an impermeable barrier. This bimodal approach to improving biostability holds great promise in the field of polyurethane biomaterials. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 108–115, 2005