Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE‐MS
Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE‐MS
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DOI：
10.1002/prot.10640
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发表时间：
2004-05
期刊：
影响因子：
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通讯作者：
Xiaobo Zhang;C. Huang;Xu-hua Tang;Ying Zhuang;C. Hew
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文献类型：
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作者：
Xiaobo Zhang;C. Huang;Xu-hua Tang;Ying Zhuang;C. Hew
白色斑点综合征病毒（WSSV）是一种主要的对虾病原体，也感染许多其他种类的甲壳类动物。其305 kb的双链DNA基因组能够编码181种推定蛋白质。为了从181种理论蛋白中鉴定病毒蛋白，通过双向电泳（2-DE）分离纯化的WSSV蛋白。通过银染法检测到60多个蛋白质点，其中12个蛋白质经质谱鉴定。总共有25种WSSV蛋白，包括我们早期研究之一中报道的那些（Huang et al.，Mol Cell Proteomics 2002;1：223-231），并且通过逆转录-聚合酶链反应（RT-PCR）进一步证实它们相应的基因。使用免疫电子显微镜将其中两个表征为WSSV包膜蛋白。我们的研究表明，蛋白质组学方法是发现病毒结构蛋白及其相应基因的有力手段。蛋白质2004;9999：000-000。© 2004 Wiley利斯公司
White spot syndrome virus (WSSV) is a major shrimp pathogen that also infects many other species of crustaceans. Its 305‐kb double‐stranded DNA genome has the capacity to encode 181 presumptive proteins. In an attempt to identify the viral proteins from the 181 theoretical proteins, proteins of the purified WSSV were separated by two‐dimensional electrophoresis (2‐DE). More than 60 protein spots were revealed, as detected by silver staining, from which 12 viral proteins were identified by mass spectrometry. In total, 25 WSSV proteins, including those reported in one of our earlier studies (Huang et al., Mol Cell Proteomics 2002;1:223–231), were revealed by this proteomic approach, and their corresponding genes were further confirmed by reverse transcription‐polymerase chain reaction (RT‐PCR). Two of them were characterized to be WSSV envelope proteins using immuno‐electron microscopy. Our study showed that the proteomic approach is a powerful method for discovering the viral structural proteins and their corresponding genes. Proteins 2004;9999:000–000. © 2004 Wiley‐Liss, Inc.