Structures of the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial Prosthetic Group
Structures of the Heme Acquisition Protein HasA with Iron(III)-5,15-Diphenylporphyrin and Derivatives Thereof as an Artificial Prosthetic Group
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DOI：
10.1002/anie.201707212
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发表时间：
2017-11-27
影响因子：
16.6
通讯作者：
Shoji, Osami
中科院分区：
文献类型：
--
作者：
Uehara, Hiromu;Shisaka, Yuma;Shoji, Osami
铁(III)-5,15-二苯基卟啉和几种衍生物被HasA容纳，HasA是铜绿假单胞菌分泌的血红素获取蛋白，尽管在卟啉的中间位置具有庞大的取代基。晶体结构分析表明，卟啉中间位置的两个苯基延伸到HasA之外。结果表明，在限铁条件下，HasA协调合成卟啉时，铜绿假单胞菌的生长受到抑制，并且合成卟啉的结构极大地影响抑制效率。
Iron(III)-5,15-diphenylporphyrin and several derivatives were accommodated by HasA, a heme acquisition protein secreted by Pseudomonas aeruginosa, despite possessing bulky substituents at the meso position of the porphyrin. Crystal structure analysis revealed that the two phenyl groups at the meso positions of porphyrin extend outside HasA. It was shown that the growth of P. aeruginosa was inhibited in the presence of HasA coordinating the synthetic porphyrins under iron-limiting conditions, and that the structure of the synthetic porphyrins greatly affects the inhibition efficiency.