Effect of drying treatment on the extractability and anti-inflammatory function of photosynthesis-related components in dulse Palmaria palmata and their efficient recovery from dried thallus
Effect of drying treatment on the extractability and anti-inflammatory function of photosynthesis-related components in dulse Palmaria palmata and their efficient recovery from dried thallus
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DOI：
10.1007/s12562-022-01619-9
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发表时间：
2022-07
影响因子：
1.9
通讯作者：
Daichi Sugita;G. Joe;Masafumi Masuoka;Y. Konishi;H. Saeki
中科院分区：
文献类型：
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作者：
Daichi Sugita;G. Joe;Masafumi Masuoka;Y. Konishi;H. Saeki
在使用含有高水分的海藻作为原材料的制造业中，海藻的体积减小是其有效储存、运输和加工的基本要求。在这项研究中，新鲜的红藻，dulsePalmaria palmata在30和60 °C下热风干燥，有或没有预冷冻1周，并使用水从干燥的菌体中提取抗炎成分，藻胆蛋白（P）和叶绿素相关化合物（C）。水提取的每一个减少预冷冻和明显受损的热空气干燥的原料dulse。相比之下，嗜热菌蛋白酶消化的P和C的混合物，这是使用脂多糖刺激的小鼠巨噬细胞评价的抗炎活性，持续尽管暴露的原料的冷冻和热空气干燥过程。此外，由热空气干燥引起的P的可提取性的劣化通过干燥硬化的细胞壁的酶降解而恢复，并且水提取物显示出强的抗炎活性。这些结果表明，热空气干燥的dulse可以用作抗炎材料，通过利用适当的物理化学程序恢复抗炎成分的可提取性，酶促藻细胞降解是这样一个实际的对策。
In manufacturing industries that use seaweeds containing high moisture as raw materials, the volume reduction of seaweeds is a basic requirement for their efficient storage, transport, and processing. In this study, fresh red algae, dulsePalmaria palmatawas hot air–dried at 30 and 60 °C with or without pre-freezing for 1 week, and anti-inflammatory components, phycobiliprotein (P) and chlorophylla-related compounds (C) were extracted together using water from the dried thalli. The water extractability of each was diminished by pre-freezing and markedly impaired by hot-air drying of the raw dulse. In contrast, the anti-inflammatory activity of thermolysin digested P and C mixtures, which was evaluated using lipopolysaccharide-stimulated murine macrophages, persisted despite exposure of the raw materials to the freezing and hot-air-drying processes. In addition, the deterioration in the extractability of P caused by hot-air drying was recovered by the enzymatic degradation of the cell wall hardened by drying, and the water extract showed strong anti-inflammatory activity. These results indicated that hot-air–dried dulse can be used as an anti-inflammatory material by utilizing the appropriate physicochemical procedure for restoring the extractability of the anti-inflammatory components, and enzymatic algal cell degradation is one such practical countermeasure.