PLASMA CAROTENOID LEVELS IN HUMAN-SUBJECTS FED A LOW CAROTENOID DIET
PLASMA CAROTENOID LEVELS IN HUMAN-SUBJECTS FED A LOW CAROTENOID DIET
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DOI：
10.1093/jn/122.1.96
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发表时间：
1992-01-01
影响因子：
4.2
通讯作者：
MCKEE, RW
中科院分区：
文献类型：
--
作者：
ROCK, CL;SWENDSEID, ME;MCKEE, RW
本研究的目的是研究低类胡萝卜素饮食对人类血浆类胡萝卜素水平的影响。作为维生素C需求研究的一部分，12名健康男性受试者在受控条件下，在一个生活代谢单元中被喂食低类胡萝卜素饮食13周。在研究第2-3、14-15、35-36和63-64天，用高效液相色谱法测定血浆类胡萝卜素（玉米黄质/叶黄素、隐黄质、番茄红素、α -胡萝卜素、β -胡萝卜素）。d 2 ~ 3 d和d 14 ~ 15 d下降速度较快，各类胡萝卜素浓度均显著降低（P < 0.05）。虽然没有更频繁的采样点就不可能得到半衰期的准确数字，但β -胡萝卜素、α -胡萝卜素和隐黄质的平均血浆耗尽半衰期似乎< 12天，番茄红素在12至33天之间，玉米黄质/叶黄素在33至61天之间。由于在研究期间下降不是线性的，这些数据表明这些化合物可能至少存在两个体库，其中一个库的周转率更快。由于血浆类胡萝卜素水平在低类胡萝卜素饮食的前两周内显著下降，因此测定这些化合物的水平可能仅在评估短期摄入量时有用。
The purpose of this study was to investigate the effect of a low carotenoid diet on plasma carotenoid levels in humans. Twelve healthy male subjects were fed a low carotenoid diet under controlled conditions for 13 wk in a live-in metabolic unit, as part of a study of vitamin C requirement. Plasma carotenoids (zeaxanthin/lutein, cryptoxanthin, lycopene, alpha-carotene, beta-carotene) were measured with HPLC on study days 2-3, 14-15, 35-36 and 63-64. The rate of decline was rapid between d 2-3 and d 14-15, when the concentration of each carotenoid decreased significantly (P < 0.05). Although accurate figures for half-life are not possible without more frequent sampling points, mean plasma depletion half-life seemed to be < 12 d for beta-carotene, alpha-carotene and cryptoxanthin, between 12 and 33 d for lycopene and between 33 and 61 d for zeaxanthin/lutein. Because the decline was not linear over the study period, these data suggest the possibility of at least two body pools of these compounds, with one pool having a more rapid turnover rate. Because there is a significant decline in plasma carotenoid levels within the first 2 wk of a low carotenoid diet, determination of levels of these compounds may be useful only in the assessment of short-term intake.