Novel calretinin and reelin expressing neuronal population includes Cajal-Retzius-type cells in the neocortex of adult pigs
Novel calretinin and reelin expressing neuronal population includes Cajal-Retzius-type cells in the neocortex of adult pigs
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DOI：
10.1016/j.neuroscience.2005.07.039
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Seress, L
中科院分区：
文献类型：
--
作者：
Abrahám, H;Tóth, Z;Seress, L
Cajal-Retzius细胞及其分泌产物reelin对大脑皮层的分层至关重要。在迄今为止研究的所有物种中，Cajal-Retzius细胞形成了一个短暂的神经元群体，出生后几乎完全从新皮层消失。最近，在成年家猪的海马结构中，我们发现了一个大的calretinin和reelin免疫反应细胞的人口，在形态学上对应于Cajal-Retzius cells.在本研究中，我们检查calretinin和reelin免疫反应神经元在第一层的前额叶，颞叶，顶叶和枕叶新皮质区的新生儿，年轻的成年和成年家猪。大量的双极或梭形钙视蛋白阳性细胞被发现在上半部分的层I在所有检查的年龄组。这些神经元的形态类似于Cajal-Retzius细胞。I层被密集的钙视蛋白阳性轴突丛所占据，这与先前描述的其他物种中Cajal-Retzius细胞的轴突相似。在一个类似的位置，其中calretinin阳性细胞发生在第一层，大量的reelin免疫反应阳性细胞被发现在所有检查的年龄组。此外，reelin共定位与calretinin在第一层神经元。从1天到1年，calretinin和reelin阳性神经元的数量减少，但calretinin阳性Cajal-Retzius型细胞仍然包括一个显着的大人口在12个月大的动物。相关的光镜和电镜检查calretinin标记的Cajal-Retzius型细胞表明，这些细胞是整合在突触回路的neocorticity.Our结果表明，Cajal-Retzius细胞并没有不可避免地消失，从成熟的新皮层在所有哺乳动物物种。这种细胞类型的功能尚不清楚，但在家猪中晚期持续存在的Cajal-Retzius型细胞提供了一个机会来研究它们的神经元连接以及reelin在新皮层可塑性和再生中的可能作用。(c)2005由Elsevier Ltd代表IBRO出版。
Cajal-Retzius cells and their secreted product reelin are essential for the lamination of the cerebral cortex. In all species studied to date Cajal-Retzius cells form a transient neuronal population that almost completely disappears from the neocortex postnatally. Recently, in the hippocampal formation of adult domestic pig, we have found a large calretinin- and reelin-immunoreactive cell population that morphologically corresponded to Cajal-Retzius cells.In the present study, we examined calretinin- and reelin-immunoreactive neurons in layer I of the prefrontal, temporal, parietal and occipital neocortical areas of newborn, young adult and adult domestic pigs. Large numbers of bipolar or fusiform calretinin-positive cells were found in the upper half of layer I in all examined age groups. The morphology of these neurons resembled that of the Cajal-Retzius cells. Layer I was occupied by a dense calretinin-positive axonal plexus that was similar to the previously described axons of Cajal-Retzius cells in other species. In a similar location, where calretinin-positive cells occurred in layer I, large numbers of reelin-immunoreactive cells were found in all examined age groups. In addition, reelin colocalized with calretinin in layer I neurons. The number of calretinin and reelin-positive neurons decreased from 1 day to one year, but calretinin-positive Cajal-Retzius-type cells still comprised a remarkable large population in 12-month-old animals. Correlated light and electron microscopic examination of calretinin-labeled Cajal-Retzius-type cells indicated that these cells are integrated in the synaptic circuitry of the neocortex.Our results suggest that Cajal-Retzius cells do not disappear inevitably from the mature neocortex in all mammalian species. The function of this cell type is not known, but late persisting Cajal-Retzius-type cells in the domestic pig provide an opportunity to study their neuronal connections and the possible role of reelin in plasticity and regeneration of neocortex. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.