Molecular phylogeography of alpine plant Metagentiana striata (Gentianaceae)
Molecular phylogeography of alpine plant Metagentiana striata (Gentianaceae)
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DOI：
10.3724/sp.j.1002.2008.07097
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发表时间：
2008-07-01
影响因子：
3.7
通讯作者：
Chen, Shi-Long
中科院分区：
文献类型：
--
作者：
Chen, Sheng-Yun;Wu, Gui-Li;Chen, Shi-Long
草本植物生命周期短，对环境变化敏感。因此，它们可以作为一个很好的模式来阐明第四纪冰期以来植物种群的历史过程。分子生物地理学分析是揭示动植物种群历史的有力工具。为了了解青藏高原高山植物的地理历史，我们调查了在整个青藏高原的Metagentianastriata（Maxim.）T. N. Ho，S. W. Liu & S. L.陈，一年生草本高山植物，分布于青藏高原及其邻近地区。通过对13个居群的155个个体的分析，共检测到7种单倍型。其中一种单倍型（A）在它们的种群中普遍存在，而三种单倍型（E、F和G）在它们的种群中是独特的。单倍型多样性中心位于青藏高原东南部的横断山区，4个居群均存在2-3种单倍型。然而，在青藏高原东北部和东部以及邻近地区的人群中，每个人群中只发现一种单倍型，不同人群具有不同的单倍型。AMOVA分析表明，种群间的遗传变异占73.05%，种群间的遗传分化估计值很高（G（ST）=0.805，F（ST）=0.731，N（ST）=0.859）。此外，各群落的地理分布也呈现出明显的异质性（N（ST）> G（ST），P
Herbaceous plants have a short life cycle and are sensitive to environmental changes. Thus, they may serve as good models to elucidate historical processes of plant populations since Quaternary glaciations. Molecular phylogeographic analysis is a powerful tool to reveal the population history of plants and animals. To understand the geographic history of alpine plants in the Qinghai-Tibet Plateau, which is still poorly known, we surveyed sequence variation of the chloroplast DNA (cpDNA) noncoding fragment trnH (GUG)-psbA intergenic spacer throughout the range of Metagentiana striata (Maxim.) T. N. Ho, S. W. Liu & S. L. Chen, an annual and herbaceous alpine plant in the Qinghai-Tibet Plateau and adjacent areas. Seven haplotypes were detected by analyzing 155 individuals from 13 populations of this species. One haplotype (A) was common and widespread, and three haplotypes (E, F and G) were unique in their populations. The center of haplotype diversity is located in the Hengduan Mountains of southeastern Qinghai-Tibet Plateau, with 2-3 haplotypes present in each of four populations. In populations from the northeastern and eastern Qinghai-Tibet Plateau as well as from adjacent regions, however, only a single haplotype was found in each population, with different populations having different haplotypes. An analysis of molecular variance (AMOVA) for populations of M striata showed that the genetic variation mainly resided among populations (73.05%), and the estimates of interpopulation differentiation were very high (G(ST)=0.805, F(ST)=0.731, N(ST)=0.859). Further, a significant phylogeographic structure was present (N(ST) > G(ST), P