MYC cis-Elements in PsMPT Promoter Is Involved in Chilling Response of Paeonia suffruticosa.
MYC cis-Elements in PsMPT Promoter Is Involved in Chilling Response of Paeonia suffruticosa.
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PsMPT启动子中的MYC顺式元件参与牡丹的低温反应
DOI：
10.1371/journal.pone.0155780
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Gai S
中科院分区：
文献类型：
--
作者：
Zhang Y;Sun T;Liu S;Dong L;Liu C;Song W;Liu J;Gai S
MPT运输Pi以合成ATP。低温诱导基因PsMPT在牡丹芽休眠解除过程中具有促进能量代谢的作用。本研究进一步分析了PsMPT启动子中参与低温反应的调控元件。PsMPT基因在牡丹不同组织中均有表达，在花瓣、柱头和雄蕊等花器官中表达量较高。通过TAIL-PCR分离了1174 bp的PsMPT启动子，并产生和分析了PsMPT启动子：：GUS转基因拟南芥。GUS染色和qPCR检测结果表明，该启动子主要在柱头和雄蕊中有活性。低温、NaCl、GA、ACC和NAA对启动子活性有促进作用，而阿坝、甘露醇和PEG对启动子活性有抑制作用。在含有PsMPT启动子421 bp的转基因植株中，低温处理显著提高了GUS基因的表达和活性。当缺失含有MYC顺式元件的-421 ~-408片段时，不能观察到低温反应。进一步的突变分析证实MYC元件是PsMPT启动子中响应低温的关键基序之一。本研究为进一步研究PsMPT在植物休眠解除过程中的调控机制提供了有用的信息。
The MPT transports Pi to synthesize ATP. PsMPT, a chilling-induced gene, was previously reported to promote energy metabolism during bud dormancy release in tree peony. In this study, the regulatory elements of PsMPT promoter involved in chilling response were further analyzed. The PsMPT transcript was detected in different tree peony tissues and was highly expressed in the flower organs, including petal, stigma and stamen. An 1174 bp of the PsMPT promoter was isolated by TAIL-PCR, and the PsMPT promoter::GUS transgenic Arabidopsis was generated and analyzed. GUS staining and qPCR showed that the promoter was active in mainly the flower stigma and stamen. Moreover, it was found that the promoter activity was enhanced by chilling, NaCl, GA, ACC and NAA, but inhibited by ABA, mannitol and PEG. In transgenic plants harboring 421 bp of the PsMPT promoter, the GUS gene expression and the activity were significantly increased by chilling treatment. When the fragment from -421 to -408 containing a MYC cis-element was deleted, the chilling response could not be observed. Further mutation analysis confirmed that the MYC element was one of the key motifs responding to chilling in the PsMPT promoter. The present study provides useful information for further investigation of the regulatory mechanism of PsMPT during the endo-dormancy release.