Method for synthesis and screening of large groups of molecularly imprinted polymers
Method for synthesis and screening of large groups of molecularly imprinted polymers
复制标题
DOI：
10.1021/ac981446p
复制
发表时间：
1999-06-01
影响因子：
7.4
通讯作者：
Sellergren, B
中科院分区：
文献类型：
--
作者：
Lanza, F;Sellergren, B
报道了一种小尺度合成分子印迹聚合物（MIPs）的技术（类似于55 mg），并结合了直接原位处理和批量重结合评价。主要的评估是基于定量的高效液相色谱法或紫外吸光度测量从给定溶剂的聚合物中释放的模板量。这种方法可以快速筛选重要的参数，以达到给定目标分子的结合亲和力和选择性的期望水平。这在三嗪类除草剂terbutylazine中得到了证明，其中对MIP制备中使用的功能单体类型进行了初步筛选。因此，在测试的六种功能单体中，甲基丙烯酸甲酯、4-乙烯基吡啶和n -乙烯基- -吡咯烷酮可以快速定量提取，而甲基丙烯酸和（三氟甲基）丙烯酸可以获得保留模板最多的聚合物。在确定了有用的功能单体后，进行了选择性的二次筛选。在此过程中，制备了非印迹空白聚合物，并进行了常规的批量再结合评价，结果表明，以甲基丙烯酸为功能单体制备的聚合物具有最高的选择性。当用色谱法对正常规模批次的聚合物进行评价时，该聚合物显示出强烈保留氯三嗪，包括阿特拉津。
A technique for the synthesis of molecularly imprinted polymers (MIPs) in small scale (similar to 55 mg) coupled with direct in situ processing and batch rebinding evaluation is reported. The primary assessment is based on quantification bq HPLC or UV absorbance measurement of the amount of template released from the polymer in a given solvent. This method allows a rapid screening of the parameters of importance to reach a desired level of binding affinity capacity and selectivity for a given target molecule. This was demonstrated for the triazine herbicide terbutylazine, where an initial screening was performed for the type of functional monomer used in the MIP preparation. Thus among the six functional monomers tested, methyl methacrylate, 4-vinylpyridine, and N-vinyl-alpha-pyrrolidone led to rapid and quantitative extraction whereas methacrylic acid and (trifluoromethyl)acrylic acid led to polymers that retained the template the most. After having established useful functional monomers, a secondary screening for selectivity was performed. In this, nonimprinted blank polymers were prepared and a normal batch rebinding evaluation was performed, The polymer showing the highest selectivity nas the one prepared using methacrylic acid as functional monomer. This polymer was shown to strongly retain chlorotriazines including atrazine when a normal-scale batch of the polymer was evaluated in chromatography.