CHEMICAL SYNTHESIS OF ACYL THIOESTERS OF ACYL CARRIER PROTEIN WITH NATIVE STRUCTURE
CHEMICAL SYNTHESIS OF ACYL THIOESTERS OF ACYL CARRIER PROTEIN WITH NATIVE STRUCTURE
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DOI：
10.1073/pnas.78.9.5440
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
KLAGES, AL
中科院分区：
文献类型：
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作者：
CRONAN, JE;KLAGES, AL
大肠杆菌的酰基载体蛋白（ACP）通过咪唑催化的N-酰基咪唑S-酰化反应转化为酰基ACP。酰化是特定的SH组，没有酰化的酪氨酸或氨基的蛋白质发生。通过凝胶电泳、疏水色谱和酶活性测定，合成的酰基-ACP底物具有天然结构。N-酰基咪唑易于合成，并允许制备那些不能酶促产生的酰基-ACP底物。
The acyl carrier protein (ACP) of Escherichia coli was converted to acyl-ACP by imidazole-catalyzed S-acylation with N-acylimidazole. The acylation was specific to the SH group; no acylation of tyrosine or amino groups of the protein occurred. The acyl-ACP substrates synthesized had a native structure as determined by gel electrophoresis, hydrophobic chromatography and enzymatic activity. N-Acylimidazoles are readily synthesized and permit preparation of those acyl-ACP substrates that cannot be produced enzymatically.