A dsRNA mycovirus causes hypovirulence of Fusarium graminearum to wheat and maize
A dsRNA mycovirus causes hypovirulence of Fusarium graminearum to wheat and maize
复制标题
DOI：
10.1007/s10658-012-9977-5
复制
发表时间：
2012-09-01
影响因子：
1.8
通讯作者：
Schaefer, Wilhelm
中科院分区：
文献类型：
--
作者：
Darissa, Omar;Adam, Guenter;Schaefer, Wilhelm
对禾谷镰刀菌分离株中国-9与dsRNA分枝病毒FgV-ch9的关联进行了低毒力相关性状的评价。源自单个分生孢子的China-9分离物的培养可能与高、中或低数量的病毒dsRNAs相关。在高和中等dsRNA水平下，China-9菌株表现出菌丝生长速度和分生能力降低，菌落形态异常，细胞质紊乱，对小麦和玉米的毒力降低。在低dsRNA水平下，真菌没有表现出任何症状，然而，RNA片段可以通过RT-PCR检测到。用纯化的FgV-ch9病毒样颗粒（VLPs）转染毒力强的F. graminearum PH-1分离物，使其对小麦和玉米的分生能力、包皮形成和致病性降低了几倍。这些结果进一步证明FgV-ch9与禾谷镰刀菌的低毒力有关。
The association of Fusarium graminearum isolate China-9 with the dsRNA mycovirus FgV-ch9 was evaluated for hypovirulence-related traits. Single conidia-originating cultures of China-9 isolate can be associated either with high, medium or low amounts of the viral dsRNAs. At high and medium dsRNA levels, China-9 isolates exhibit reduced mycelia growth rate and conidiation capacity, abnormal colony morphology, disorganized cytoplasm, as well as reduced virulence for wheat and maize plants. At low dsRNA levels the fungus shows no symptoms, however, the RNA segments can be detected by RT-PCR. Transfection of the virulent F. graminearum PH-1 isolate with purified Virus-like Particles (VLPs) of FgV-ch9 reduced its conidiation capacity, perithecia formation, and pathogenicity for wheat and maize several folds. These results further demonstrate that FgV-ch9 is associated with hypovirulence of F. graminearum.