Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori:: correlation with ecdysteroid secretion
Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori:: correlation with ecdysteroid secretion
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DOI：
10.1016/s0965-1748(00)00127-2
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发表时间：
2001-03-15
影响因子：
3.8
通讯作者：
Kataoka, H
中科院分区：
文献类型：
--
作者：
Mizoguchi, A;Ohashi, Y;Kataoka, H
建立了一种灵敏的、时间分辨的家蚕促胸激素（pth）荧光免疫测定方法。本方法检测下限为0.1 pg。采用本方法定量测定中枢神经系统和血淋巴中甲状旁腺激素的含量。甲状旁腺激素仅在中枢神经系统内的大脑中检测到，在第5龄时，随着幼虫的生长，其在大脑中的含量逐渐增加，在开始流浪后迅速下降。在第3天的5龄幼虫的脑后复合体中也发现了大量的甲状旁腺激素，占总甲状旁腺激素的28%。血淋巴中甲状旁腺素滴度在家蚕发育过程中变化剧烈，在四龄中期有一个小峰值，在五龄徘徊期和蛹前期有一个中等峰值，在蛹-成虫发育早期有一个大的延长峰。这些变化总体上与血淋巴表皮类固醇滴度密切相关。然而，血淋巴中PTTH动力学的一些意想不到的方面也被揭示出来。在此基础上，讨论了甲状旁腺激素分泌在控制昆虫发育中的意义。2001爱思唯尔科学有限公司版权所有。
A very sensitive time-resolved fluoroimmunoassay for the prothoracicotropic hormone (PTTH) of the silkworm Bombyx mori has been established. The lower limit of detection in this assay was 0.1 pg. With this assay method, the amounts of PTTH in the central nervous system and hemolymph were quantified. PTTH was detected only in the brain within the central nervous system, and, in the fifth instar, its content in the brain increased gradually with larval growth and decreased rapidly after the beginning of wandering. A substantial amount of PTTH was also found in the retrocerebral complex of day-3 fifth instar larvae, accounting for 28% of total PTTH. The PTTH titer in hemolymph changed dramatically during Bombyx development, with a small peak in the middle of the fourth instar, medium-sized peaks at the wandering and prepupal stages in the fifth instar, and a large prolonged peak during early pupal-adult development. The changes were overall closely correlated with those in hemolymph ecdysteroid titer. However, some unexpected aspects of PTTH dynamics in hemolymph have also been disclosed. Based on these observations, the significance of PTTH secretion in the control of insect development is discussed. (C) 2001 Elsevier Science Ltd. All rights reserved.