Inhibitory effect of local anaesthetics on reactive oxygen species production by human neutrophils
Inhibitory effect of local anaesthetics on reactive oxygen species production by human neutrophils
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DOI：
10.1111/j.1399-6576.1997.tb04735.x
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发表时间：
1997-04-01
影响因子：
2.1
通讯作者：
Niwa, Y
中科院分区：
文献类型：
--
作者：
Mikawa, K;Akamatsu, H;Niwa, Y
背景资料：中性粒细胞产生的活性氧（ROS）在机体各主要器官中的蓄积被认为在机体自身损伤的发病机制中起着关键作用。利多卡因已被证明可以减轻损伤。我们研究了局部麻醉剂（利多卡因，甲哌卡因和布比卡因）对活性氧生产的中性粒细胞使用在体外system.Methods的影响：我们测量了生产超氧化物（铁细胞色素c法），过氧化氢（H2 O2：东莨菪碱荧光技术），和羟基自由基（OH .：乙烯气体法）在不存在和存在利多卡因（2-200 μ g/mL）、甲哌卡因（3-300 μ g/mL）和布比卡因（3- 300 μ g/mL）的情况下，通过从人类成年志愿者分离的中性粒细胞进行。我们还测量了在无细胞（黄嘌呤-黄嘌呤氧化酶）system.Results的ROS生成：利多卡因和甲哌卡因在较高的水平显着降低了由中性粒细胞的ROS的生产。然而，临床相关血液浓度的这些局部麻醉剂对ROS水平没有影响。此外，利多卡因和甲哌卡因未能减少由无细胞系统产生的ROS。布比卡因没有减少ROS的产生，无论是生成system.Conclusion：总之，在目前的体外系统中，只有浓度的利多卡因和甲哌卡因100倍以上的临床可行的人中性粒细胞减少ROS的生产。然而，临床相关血药浓度的局部麻醉剂无抑制作用。需要使用体内系统进行进一步研究，以阐明局部麻醉剂在临床环境中对ROS生成的抑制作用。(C)斯堪的纳维亚麻醉学学报41（1997年）。
Background: Reactive oxygen species (ROS) generated from neutrophils accumulated in various major organs are thought to play a pivotal role in the pathogenesis of host auto-injury. Lidocaine has been shown to reduce the injury. We investigated the effect of local anaesthetics (lidocaine, mepivacaine and bupivacaine) on ROS production by neutrophils using an in vitro system.Methods: We measured the production of superoxide (ferric ytochrome c method), hydrogen peroxide (H2O2: scopoletin fluorescence technique), and hydroxyl radical (OH .: ethylene gas method) by neutrophils isolated from human adult volunteers in the absence and presence of lidocaine (2-200 mu g/mL), mepivacaine (3-300 mu g/mL), and bupivacaine (3-300 mu g/mL). We also measured the ROS generation in a cell-free (xanthine-xanthine oxidase) system.Results: Lidocaine and mepivacaine at higher levels significantly decreased the production of ROS by neutrophils. However, these local anaesthetics at clinically relevant blood concentrations had no effect on the levels of ROS. Furthermore, lidocaine and mepivacaine failed to reduce ROS generated by the cell-free system. Bupivacaine did not decrease ROS generation by either generating system.Conclusion: In conclusion, in the present in vitro system, only concentrations of lidocaine and mepivacaine 100-fold higher than clinically feasible ones reduced ROS production by human neutrophils. However, the local anaesthetics at clinically relevant blood concentrations had no suppressive effect. Further studies using in vivo systems are required to elucidate the inhibitory effects of local anaesthetics on ROS generation in clinical settings. (C) Acta Anaesthesiologica Scandinavica 41 (1997).