Stripe artifact reduction for digital scanned structured illumination light sheet microscopy
Stripe artifact reduction for digital scanned structured illumination light sheet microscopy
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DOI：
10.1364/ol.44.002510
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发表时间：
2019-05-15
期刊：
影响因子：
3.6
通讯作者：
Kner, Peter
中科院分区：
文献类型：
--
作者：
Liu, Yang;Lauderdale, James D.;Kner, Peter
光片显微镜是研究大型和半不透明生物样品的一种重要而广泛使用的方法。该方法的一个缺点是，由于照明路径中的吸收和散射，它经常导致条纹伪影。在这里，我们描述了一种新的方法，它将有效地消除数字扫描光片显微镜(DSLM)和数字扫描结构照明光片显微镜(DSLM-SI)中的伪影。我们通过一种新的重建方法进一步改善了DSLM-SI的结果，获得了更清晰的重建图像。我们通过成像156微米深入斑马鱼幼体中枢神经系统来证明条纹伪影的减少。条带伪影的大小在三个数据集中平均减少了20%。(C)2019年美国光学学会
Light sheet microscopy is an important and widely used method for studying large and semi-opaque biological specimens. One drawback of the approach is that it often results in stripe artifacts due to absorption and scattering in the illumination path. Here we describe a new approach which will effectively mitigate the artifacts in digital scanned light sheet microscopy (DSLM) and digital scanned structured illumination light sheet microscopy (DSLM-SI). We further improve the results of DSLM-SI through a new reconstruction method which achieves clearer reconstructed images. We demonstrate the reduction of stripe artifacts by imaging 156 microns deep into the larval zebrafish central nervous system. The magnitude of stripe artifacts is reduced by an average of 20% across three datasets. (C) 2019 Optical Society of America