Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.
Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.
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DOI：
10.1038/srep13412
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发表时间：
2015-08-21
影响因子：
4.6
通讯作者：
Sahoo DK
中科院分区：
文献类型：
--
作者：
Chopra L;Singh G;Kumar Jena K;Sahoo DK
抗生素抗药性细菌的出现导致了对替代治疗药物的探索，例如被称为细菌素的核糖体合成细菌肽。生物膜是导致严重慢性感染的微生物群落，形成的环境可以增强抗菌素的耐药性。生物膜中的细菌对抗生素的抗药性可能比浮游状态下循环的相同细菌高出数千倍。在这项研究中，Sonorensin被预测属于细菌素的杂环炭疽亚家族，被发现能有效地杀死革兰氏阳性和革兰氏阴性细菌的活性细胞和非增殖细胞。Sonorensin对金黄色葡萄球菌生物被膜有明显的抑制作用。荧光和电子显微镜显示，生长抑制是由于膜透性增加所致。在低密度聚乙烯膜上涂覆索诺菌素，可以有效地抑制单核细胞增生性李斯特菌和金黄色葡萄球菌等食品腐败菌的生长。Sonorensin涂层对鸡肉和番茄样品中腐败菌的生长具有抑制作用，表明Sonorensin作为现有抗生素/防腐剂的替代品具有潜在的潜力。
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.