Matrix Assisted Rapid Evaporative Ionization Mass Spectrometry
Matrix Assisted Rapid Evaporative Ionization Mass Spectrometry
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DOI：
10.1021/acs.analchem.9b01441
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发表时间：
2019-08-06
影响因子：
7.4
通讯作者：
Takats, Zoltan
中科院分区：
文献类型：
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作者：
Jones, Emrys A.;Simon, Daniel;Takats, Zoltan
快速蒸发电离质谱（REIMS）是一种高度通用的技术，允许对一系列生物固体或液体样品进行采样，而无需样品制备。这种直接方法的代价是某些样品类型只能提供中等数量的化学信息。在这里，我们介绍了一个矩阵辅助版本的技术（MA-REIMS），其中的气溶胶的纯溶剂，如异丙醇，与样品的快速蒸发所产生的样品气溶胶混合，它被证明是提高信号强度从一个REIMS采样事件超过2个数量级。这种增加极大地扩展了该技术的范围，同时提供了额外的益处，例如减少REIMS源的结垢，并允许恒定引入校准校正化合物的简单方法以进行精确的质量测量。一系列的实验，以调查发生在这种修改后的方法的过程中，这种增强是至关重要的，如手术内组织识别的应用程序，进行了讨论。
Rapid evaporative ionization mass spectrometry (REIMS) is a highly versatile technique allowing the sampling of a range of biological solid or liquid samples with no sample preparation. The cost of such a direct approach is that certain sample types provide only moderate amounts of chemical information. Here, we introduce a matrix assisted version of the technique (MA-REIMS), where an aerosol of a pure solvent, such as isopropanol, is mixed with the sample aerosol generated by rapid evaporation of the sample, and it is shown to enhance the signal intensity obtained from a REIMS sampling event by over 2 orders of magnitude. Such an increase greatly expands the scope of the technique, while providing additional benefits such as reducing the fouling of the REIMS source and allowing for a simple method of constant introduction of a calibration correction compound for accurate mass measurements. A range of experiments are presented in order to investigate the processes that occur within this modified approach, and applications where such enhancements are critical, such as intrasurgical tissue identification, are discussed.