Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging
Synthesis and Characterization of Oligofluorene Nanoparticles for Cell Imaging
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DOI：
10.6023/a12070461
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发表时间：
2012-10
影响因子：
2.5
通讯作者：
Quanshan Wen;Hongwei Tang;Gaomai Yang;Libing Liu;Fengting Lv;Y. Qiong;Wang Shu
中科院分区：
文献类型：
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作者：
Quanshan Wen;Hongwei Tang;Gaomai Yang;Libing Liu;Fengting Lv;Y. Qiong;Wang Shu
通过Suzuki交叉偶联反应合成了一种新型疏水低芴。在高摩尔吸收系数（1.08 × 105mol - 1•L•cm - 1）和荧光量子产率（0.96）的氯仿中测量了吸收光谱和荧光光谱。通过动态光散射（DLS）测量，将OF分散到水中形成平均粒径为230 nm的纳米颗粒。纳米粒子在水中仍然具有较高的摩尔吸收系数和荧光量子产率。采用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）法测定了of纳米颗粒对人肺癌（A549）细胞的定量细胞毒性，结果表明of纳米颗粒具有较低的细胞毒性。OF纳米颗粒可用于A549活细胞的成像。共聚焦激光扫描显微镜显示OF纳米颗粒仅位于细胞质中，特别是在核周区域周围。OF纳米颗粒与溶酶体特异性染料（Lyso Tracker Red）表现出良好的重叠染色，表明OF纳米颗粒可用于溶酶体特异性成像。
A new hydrophobic oligofluorene ( OF ) is synthesized via Suzuki cross-coupling reaction. The absorption and fluorescence spectra are measured in chloroform with high molar absorption coefficient (1.08 × 10 5 mol － 1 • L•cm － 1 ) and fluorescence quantum yield (0.96). OF is dispersed into water to form nanoparticles with average size of 230 nm from dynamic light scattering (DLS) measurement. The nanoparticles in water still possess high molar absorption coefficient and fluorescence quantum yield. The quantitative cytotoxicity of OF nanoparticles to human lung cancer (A549) cells is measured by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, showing that OF nanoparticles exhibit low cellular toxicity. OF nanoparticles can be utilized for imaging of live A549 cells. Confocal laser scanning microscopy demonstrates that OF nanoparticles are exclusively located in the cytoplasm, especially around the perinuclear region. OF nanoparticles exhibit good overlap staining with lysosome-specific dye (Lyso Tracker Red), which demonstrates that OF nanoparticles could be used for lysosome-specific imaging.