Using Morpholinos to Control Gene Expression.
Using Morpholinos to Control Gene Expression.
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DOI：
10.1002/cpnc.21
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发表时间：
2017-03-02
影响因子：
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通讯作者：
Moulton, Jon D
中科院分区：
文献类型：
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作者：
Moulton, Jon D
吗啉代寡核苷酸是稳定的、不带电荷的水溶性分子，用于阻断RNA的互补序列，防止在这些位点处的加工、通读或蛋白质结合。吗啉代通常用于阻断mRNA的翻译和阻断前mRNA的剪接，尽管它们可以阻断生物大分子和RNA之间的其他相互作用。吗啉代是有效的，特异性的，并且缺乏非反义作用。它们在任何转录和翻译RNA的细胞中起作用，但必须被递送到核/胞质区室才能有效。吗啉代与互补核酸序列形成稳定的碱基对，但显然不与蛋白质结合到显著的程度。它们不被任何蛋白质识别，也不经历蛋白质介导的催化，也不介导RNA酶H或RISC复合物对RNA的切割。这项工作的重点是使用Morpholinos的技术和背景。John Wiley & Sons，Inc.
Morpholino oligonucleotides are stable, uncharged, water-soluble molecules used to block complementary sequences of RNA, preventing processing, read-through, or protein binding at those sites. Morpholinos are typically used to block translation of mRNA and to block splicing of pre-mRNA, though they can block other interactions between biological macromolecules and RNA. Morpholinos are effective, specific, and lack non-antisense effects. They work in any cell that transcribes and translates RNA, but must be delivered into the nuclear/cytosolic compartment to be effective. Morpholinos form stable base pairs with complementary nucleic acid sequences but apparently do not bind to proteins to a significant extent. They are not recognized by any proteins and do not undergo protein-mediated catalysis-nor do they mediate RNA cleavage by RNase H or the RISC complex. This work focuses on techniques and background for using Morpholinos. © 2017 by John Wiley & Sons, Inc.